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DOI :
10.3791/50730-v
October 15th, 2013
Chapters
0:05
Title
1:08
Protein Extraction
2:41
First-dimensional Isoelectric Focusing
4:06
Second-dimension SDS Polyacrylamide Gel Electrophoresis and Sliver Staining
7:14
Representative Protein Extraction Results
7:58
Conclusion
バークホルデリア属のメンバーは、臨床的に重要な病原体である。我々は、機械的破壊、およびその後のプロテオーム解析のための2-Dゲル電気泳動を用いて、全細菌タンパク質抽出するための方法を記載している。
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