JoVE Logo
Faculty Resource Center

Sign In

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

DOI :

10.3791/50733-v

November 26th, 2013

November 26th, 2013

13,998 Views

1University of Victoria-Genome BC Proteomics Centre, University of Victoria, 2Department of Biochemistry and Microbiology, University of Victoria

Dithranol (DT; 1,8-dihydroxy-9,10-dihydroanthracen-9-one) has previously been reported as a MALDI matrix for tissue imaging of small molecules; protocols for the use of DT for the MALDI imaging of endogenous lipids on the surface of tissue sections by positive-ion MALDI-MS on an ultrahigh-resolution quadrupole-FTICR instrument are provided here.

Tags

Dithranol

-- Views

Related Videos

article

MALDI Sample Preparation: the Ultra Thin Layer Method

article

Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels

article

Quantifying Mixing using Magnetic Resonance Imaging

article

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

article

Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening

article

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

article

Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

article

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

article

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

article

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved