JoVE Logo
Faculty Resource Center

Sign In

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

DOI :

10.3791/50765-v

September 26th, 2013

September 26th, 2013

33,719 Views

1Department of Microbiology and Cell Science, The University of Florida

With effector functions distinct from other T cell subsets, Th17 cells have been centrally implicated in inflammatory autoimmunity. This in vitro Th17 differentiation protocol provides a means to determine whether naïve CD4+ T lymphocytes can differentiate into Th17 cells, and to further examine their role in autoimmunity and host response.

Tags

Isolation

-- Views

Related Videos

article

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

article

Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes

article

Isolation of Lymphocytes from Mouse Genital Tract Mucosa

article

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

article

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

article

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

article

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

article

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

article

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor

article

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved