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DOI :
10.3791/50830-v
October 11th, 2013
Chapters
0:05
Title
1:37
Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
2:40
Transfection of Mammalian Cells
3:48
Co-culture of Mammalian Cells with Avian Cells
5:22
Infection of Chicken Embryonated Eggs
6:31
Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
7:52
Hemagglutination (HA) Assay
9:03
Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
10:33
Conclusion
ニューカッスル病ウイルス(NDV)は広く、とりわけ、ワクチン接種および治療のための新たなベクターを開発するために、ここ数年で研究されてきた。これらの研究は、そのような私たちがここで説明しているようなcDNAから組換えウイルスを救出するための技術、することが可能となっている。
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