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13.4K Views
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11:10 min
November 27th, 2013
DOI :
10.3791/50861-v
Chapters
0:05
Title
1:18
Brain Slicing and Dissection
2:35
Dissociation and Culture
5:50
Preparation for Membrane Potential Dye Imaging
8:23
MPD Imaging
9:36
Results: Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons
10:43
Conclusion
Summary
从成年鼠龄的单神经元的活性可以通过从特定脑区域的神经元解离,并使用荧光膜电位染料成像进行研究。通过测试响应变化的葡萄糖,这种技术可以用来研究成年腹内侧下丘脑神经元的葡萄糖敏感性。
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