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Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

13.6K Views

11:10 min

November 27th, 2013

November 27th, 2013

13,624 Views

0:05

Title

1:18

Brain Slicing and Dissection

2:35

Dissociation and Culture

5:50

Preparation for Membrane Potential Dye Imaging

8:23

MPD Imaging

9:36

Results: Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons

10:43

Conclusion

The activity of single neurons from adult-aged mice can be studied by dissociating neurons from specific brain regions and using fluorescent membrane potential dye imaging. By testing responses to changes in glucose, this technique can be used to study the glucose sensitivity of adult ventromedial hypothalamic neurons.

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