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A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

DOI :

10.3791/50908-v

December 27th, 2013

December 27th, 2013

5,217 Views

1Department of Chemistry, The Scripps Research Institute, 2Worm Institute of Research and Medicine (WIRM), The Scripps Research Institute

The botulinum neurotoxin type A light chain (BoNT/A LC) is a metalloprotease that enters motor neurons, cleaves its substrate SNAP-25, and disrupts neurotransmission, thereby resulting in flaccid paralysis. Utilizing a high-throughput-compatible FRET-based assay, large libraries of small molecules can be screened for their impact on BoNT/A LC enzymatic activity.

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