Name: ascending aortic constriction in rats for creation of pressure overload cardiac hypertrophy model
Uploaded: 2014-06-29T14:00:00
Description: Watch this Scientific Journal Video about Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model at JoVE.com

作者承认生物技术系，印度政府为这个项目提供财政支持。阿吉特·库马尔GS与科学和工业研究，印度政府和Binil拉吉与医学研究，印度政府的印第安人理事会高级研究奖学金理事会高级研究奖学金得到了支持。

动物实验获得学院动物伦理委员会批准后执行。 Wistar大鼠容纳下一个12小时黑暗的光周期，恒定室温（24±2℃）与食物和水随意（按照CPCSEA准则）。 1）手术前护理和麻醉<ol><li>根据抗生素伞24小时保持大鼠（约200g体重）术前通过口服阿莫西林（剂量：水500毫克/升）。 </li><li>通过在121℃下高压灭菌15分钟进行消毒所有外科工具（如剪刀，镊子，夹子施放器，缝合针和缝合针?...

<ol>
	<li>Patten, R. D., Hall-Porter, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small+animal+models+of+heart+failure:+development+of+novel+therapies,+past+and+present.">Small animal models of heart failure: development of novel therapies, past and present.</a> Circ Heart Fail. 2, 138-144 (2009).</li><li>Weinberg, E. O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Angiotensin-converting+enzyme+inhibition+prolongs+survival+and+modifies+the+transition+to+heart+failure+in+rats+with+pressure+overload+hypertrophy+due+to+ascending+aortic+stenosis.">Angiotensin-converting enzyme inhibition prolongs survival and modifies the transition to heart failure in rats with pressure overload hypertrophy due to ascending aortic stenosis.</a> Circulation. 90, 1410-1422 (1994).</li><li>Schunkert, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Increased+rat+cardiac+angiotensin+converting+enzyme+activity+and+mRNA+expression+in+pressure+overload+left+ventricular+hypertrophy.+Effects+on+coronary+resistance,+contractility,+and+relaxation.">Increased rat cardiac angiotensin converting enzyme activity and mRNA expression in pressure overload left ventricular hypertrophy. Effects on coronary resistance, contractility, and relaxation.</a> J Clin Invest. 86, 1913-1920 (1990).</li><li>Hasenfuss, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+human+cardiovascular+disease,+heart+failure+and+hypertrophy.">Animal models of human cardiovascular disease, heart failure and hypertrophy.</a> Cardiovasc Res. 39, 60-76 (1998).</li><li>Litwin, S. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Serial+echocardiographic-Doppler+assessment+of+left+ventricular+geometry+and+function+in+rats+with+pressure-overload+hypertrophy.+Chronic+angiotensin-converting+enzyme+inhibition+attenuates+the+transition+to+heart+failure.">Serial echocardiographic-Doppler assessment of left ventricular geometry and function in rats with pressure-overload hypertrophy. Chronic angiotensin-converting enzyme inhibition attenuates the transition to heart failure.</a> Circulation. 91, 2642-2654 (1995).</li><li>Miyamoto, M. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adenoviral+gene+transfer+of+SERCA2a+improves+left-ventricular+function+in+aortic-banded+rats+in+transition+to+heart+failure.">Adenoviral gene transfer of SERCA2a improves left-ventricular function in aortic-banded rats in transition to heart failure.</a> Proc Natl Acad Sci U S A. 97, 793-798 (2000).</li><li>Kagaya, Y., Hajjar, R. J., Gwathmey, J. K., Barry, W. H., Lorell, B. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-term+angiotensin-converting+enzyme+inhibition+with+fosinopril+improves+depressed+responsiveness+to+Ca2++in+myocytes+from+aortic-banded+rats.">Long-term angiotensin-converting enzyme inhibition with fosinopril improves depressed responsiveness to Ca2+ in myocytes from aortic-banded rats.</a> Circulation. 94, 2915-2922 (1996).</li><li>Del Monte, F., Butler, K., Boecker, W., Gwathmey, J. K., Hajjar, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Novel+technique+of+aortic+banding+followed+by+gene+transfer+during+hypertrophy+and+heart+failure.">Novel technique of aortic banding followed by gene transfer during hypertrophy and heart failure.</a> Physiol Genomics. 9, 49-56 (2002).</li><li>Turcani, M., Rupp, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+failure+development+in+rats+with+ascending+aortic+constriction+and+angiotensin-converting+enzyme+inhibition.">Heart failure development in rats with ascending aortic constriction and angiotensin-converting enzyme inhibition.</a> Br J Pharmacol. 130, 1671-1677 (2000).</li><li>Kerem, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lung+endothelial+dysfunction+in+congestive+heart+failure:+role+of+impaired+Ca2++signaling+and+cytoskeletal+reorganization.">Lung endothelial dysfunction in congestive heart failure: role of impaired Ca2+ signaling and cytoskeletal reorganization.</a> Circ Res. 106, 1103-1116 (2010).</li><li>Almeida, A. C., van Oort, R. J., Wehrens, X. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transverse+aortic+constriction+in+mice.">Transverse aortic constriction in mice.</a> J Vis Exp. (38), (2010).</li></ol>

有几种手术的型号为感应压力超负荷以及心肌肥厚和心脏衰竭。在小动物，Lorell和他的同事开发了我们在这里所描述的模型，他们报告的代偿性心肌肥厚后8周主动脉缩窄5的发生。心脏的变化逐渐进展到失代偿状态，然后导致心脏衰竭。这种模式的主要优点是逐步发病增加，左心室压力，从而类似于在慢性高血压的临床情况的。它也是一种合适的动物模型用于研究相关 ​​的左心脏衰竭?...

小动物模型是用于在心脏肥大及其进展为心脏衰竭的发展慢性变化的研究最优选的工具。通过手术方法心脏衰竭模型最初是在大鼠为这些动物都是敞胸外科手术，超声心动图血流动力学参数和评估最合适。它们还提供了充足的验尸样本进行组织，细胞和分子水平的研究1。升主动脉缩窄是最常见的和成功的手术模型用于创建压力超负荷心脏衰竭2，3中的一个。这种模式是非常适合从肥大心脏衰竭4在过渡期间，研究细胞和亚细胞重塑事件和心脏的收缩性能。 这种模式的主要优点是逐渐发生的5心脏压力超负荷，&lt;/燮&gt; 6。这种模式是因为其缓慢但稳定的进展从补偿心肌肥厚到失代偿阶段，最后到心脏衰竭阶段临床相关性。持续时间为心肌肥厚心脏衰竭和相关生理变化的进程主要受狭窄程度产生7。升主动脉缩窄可以通过两种方式来进行，或者通过使用缝合线或者由应用的金属夹子8,9。使用金​​属夹，用于主动脉缩窄的主要优点是，外科手术是不太复杂的。在该方法中人们必须找出同上瓣膜升主动脉，插入片段围绕主动脉取得缩颈的期望的水平。胸部牵开器是没有必要在此过程。主动脉缩窄通过缝合需要更多的手术操作用于放置针头升主动脉旁，周围缝合到产生主动脉缩窄的所需的水平。后者的技术是更多的时间时，与使用金属夹子对主动脉缩窄比较耗时。在这个视频中描述的方法演示了外科手术升大鼠主动脉缩窄，使压力超负荷创造左心室肥厚模型。

<table border="0" cellpadding="0" cellspacing="0" width="742">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Wistar rats</td>
			<td style="width:149px;"></td>
			<td style="width:119px;"></td>
			<td style="width:220px;">Maintained at ARF, RGCB</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Inhalation anaesthesia systems&#160;</td>
			<td style="width:149px;">VetEquip</td>
			<td style="width:119px;">AB19276</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Rodent ventilator</td>
			<td style="width:149px;">CWE Inc</td>
			<td style="width:119px;">SAR- 830/AP&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Rat tracheal cannula</td>
			<td style="width:149px;">CWE Inc</td>
			<td style="width:119px;">13-21032</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Ultrasound system</td>
			<td style="width:149px;">Philips</td>
			<td style="width:119px;">HD7</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Ultrasound transducer</td>
			<td style="width:149px;">Philips</td>
			<td style="width:119px;">S 12</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Titanium clip (small)</td>
			<td style="width:149px;">Horizon</td>
			<td style="width:119px;">1204</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Clip applicator</td>
			<td style="width:149px;">Weck</td>
			<td style="width:119px;">137081</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Electric shaver</td>
			<td style="width:149px;">Vinverth</td>
			<td style="width:119px;">VBT0817</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">BP blade (size:11)</td>
			<td style="width:149px;">Surgeon</td>
			<td style="width:119px;">REF10111</td>
			<td>Preparation of surgical site</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Tramadol</td>
			<td style="width:149px;">Orchid health care</td>
			<td style="width:119px;">OCH043</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Amoxycillin Hydrochloride</td>
			<td style="width:149px;">Ranbaxy</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Isoflurane</td>
			<td style="width:149px;">Piramal health care</td>
			<td style="width:119px;">A23M10A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Syringes</td>
			<td style="width:149px;">BD</td>
			<td style="width:119px;">2015-09</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">4-0 braided silk</td>
			<td style="width:149px;">Diamond</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">3-0 prolene</td>
			<td style="width:149px;">Johnson&#38;Johnson</td>
			<td style="width:119px;">NW018</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Surgical tape</td>
			<td style="width:149px;">Romsons</td>
			<td style="width:119px;">SH 6301</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Povidone iodine</td>
			<td style="width:149px;">Win Medicare</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:255px;">Temp. controlled heating pad</td>
			<td style="width:149px;">Flamingo</td>
			<td style="width:119px;">HC1003</td>
			<td></td>
		</tr>
	</tbody>
</table>

ascending aortic constriction in rats for creation of pressure overload cardiac hypertrophy model

升主动脉缩窄是一个用于创建诱导的心肌肥厚和心脏衰竭压力超负荷最常见和最成功的手术模型。在这里，我们描述了使用一个小的金属夹子由升主动脉缩窄建立压力负荷和心肌肥厚大鼠详细的手术过程。麻醉后，气管是通过一个半路上切口气管2软骨环之间进行插入插管插管。然后一个皮肤切口，在第二肋间水平上的左胸壁和肌肉层被清除，以找出主动脉的上升部分。升主动脉的收缩到其原始直径由应用的小尺寸钛夹的50-60％。以下主动脉缩窄，第二和第三肋近似prolene线缝合。气管插管被删除，一旦自主呼吸被重新建立。动物被允许恢复Ø通过逐渐降低麻醉n为加热垫。通过升主动脉的收缩产生的压力超负荷的强度是通过使用反式胸二维多普勒超声心动图记录的压力梯度来确定。总体来说，这一协议是逐渐发生和发展的代偿性心肌肥厚心脏衰竭阶段时，研究重塑事件和心脏的收缩特性非常有用。

在我们的实验中，我们能够实现80％以上的存活率。主动脉缩窄的效果是通过执行多普勒超声心动图证实。大鼠（N = 6），其在主动脉狭窄部位约60毫米汞柱的压力梯度，观察8周，处死以便分析心肌肥大的发展（ 图1）。 8周后手术后的超声心动图评价结果显示左心室参数，如跨室间隔厚度，左室后壁厚度及减少左室内径在舒张状态，动物，进行主动脉缩窄显著增加，较假手术对照组（...

Watch this Scientific Journal Video about Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model at JoVE.com

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

大鼠建立压力超负荷心肌肥厚模型在升主动脉缩窄

我们描述了使用一个小的金属夹子由升主动脉缩窄建立压力负荷和左心室肥厚大鼠一个逐步的过程。该模型被广泛用于心肌肥厚过程中学习重塑变化，物色及评估策略，这种变化的回归。

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

Research

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Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography

右心室结构和功能的肺动脉缩窄经胸超声小鼠模型的评估

Emerging clinical data support the notion that RV dysfunction is critical to the pathogenesis of cardiovascular disease and heart failure1-3. Moreover, ...

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Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

测量升主动脉刚度<em&gt;在体内</em&gt;小鼠使用超声

We present a protocol for measuring in vivo aortic stiffness in mice using high-resolution ultrasound imaging. Aortic diameter is measured by ultrasound ...

Ultrasound Based Assessment of Coronary Artery Flow and Coronary Flow Reserve Using the Pressure Overload Model in Mice

冠脉流量和冠状动脉血流储备用小鼠压力超负荷基于模型的超声评估

Transthoracic Doppler echocardiography (TTDE) is a clinically useful, noninvasive tool for studying coronary artery flow velocity and coronary flow ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

在横向主动脉缩窄的小鼠模型和心脏形态学评估功能改变通过超声心动图成像

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats

心脏重构通过大鼠腹主动脉缩窄的模型

Heart failure is one of the leading causes of death worldwide. It is a complex clinical syndromethat includes fatigue, dyspnea, exercise intolerance, and ...

Minimally Invasive Transverse Aortic Constriction in Mice

微创横向主动脉缩窄小鼠

Minimally invasive transverse aortic constriction (MTAC) is a more desirable method for the constriction of the transverse aorta in mice than standard ...

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy

小鼠横主动脉狭窄诱导左心室肥厚的技术研究

Transverse aortic constriction (TAC) in mice is one of the most commonly used surgical techniques for experimental investigation of pressure ...

A Closed-chest Model to Induce Transverse Aortic Constriction in Mice

诱导小鼠横向主动脉狭窄的闭合胸模型

Research on cardiac hypertrophy and heart failure is frequently based on pressure overload mouse models induced by TAC. The standard procedure is to ...