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JoVE Journal

Medicine

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Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

16.9K Views

10:18 min

June 29th, 2014

June 29th, 2014

16,902 Views

0:00

Title

0:06

Introduction

2:30

Pre-Operative Care and Anesthesia

2:43

Preparation of the Surgical Site

3:22

Tracheal Intubation in Rat

4:05

Constriction of Ascending Aorta

7:05

Post-operative Care

7:58

Confirmation of Successful Constriction of Ascending Aorta

8:14

Representative Results

9:21

Conclusion

We describe a stepwise procedure for creating pressure overload and left ventricular hypertrophy in Wistar rats by constriction of the ascending aorta using a small metallic clip. This model is extensively used for studying remodeling changes during cardiac hypertrophy and for identifying and evaluating strategies for regression of such changes.

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