Name: a novel light damage paradigm for use in retinal regeneration studies in adult zebrafish
Uploaded: 2013-10-24T15:50:00
Description: Watch this Scientific Journal Video about A Novel Light Damage Paradigm for Use in Retinal Regeneration Studies in Adult Zebrafish at JoVE.com

笔者想感谢西峡罗优良的鱼类养殖和技术支持。这项工作是由美国国立卫生赠款R21EY019401（RT）和P30EY04068（RT），和启动资金RT，包括无限制的授予从韦恩州立大学，眼科防盲研究。 JT是由托马斯·C.隆隆韦恩州立大学研究生院提供的奖学金支持。

在韦恩州立大学医学院动物使用委员会批准，在本协议中所述的所有程序。 1。暗适应<ol><li>转让〜10成年白化或色素的鱼，从正常的住房制度进入一个黑暗的外壳。如果可用，建成斑马鱼的正常水流通过水箱外壳模块，它允许使用一个黑暗的外壳。 （如果这样的系统不可用，放置鱼缸在一个完全黑暗的外壳，确保通气鱼与氧气）。 </li><li>保持10天的鱼在黑暗中。当?...

在这里，我们提供持续的强光曝晒普遍的感光损失和强大的再生反应的结果，结合短的紫外线照射。这个相结合的方法相比与个人LIRD方法，也是最有效的协议，损害双方的两部分的视网膜杆和视锥。重要的是，这种治疗是有效的颜料的动物，以及患白化病的动物。 虽然我们提供的证据表明与个别LIRD方法相比更为广泛和一致的损伤合并协议结果，科学的考虑，应该讨论...

光诱导的视网膜变性（LIRD）是常用的啮齿动物损伤杆和视锥细胞和斑马鱼。在成年斑马鱼，光感受器变性触发穆勒胶质细胞重新进入细胞周期和产生瞬态放大祖​​。这些祖细胞继续增殖，因为他们迁移到受损区域，在那里他们最终产生新的光感受器。目前，有两个广泛使用LIRD的范例，其中每个感光损失和相应的差异在再生反应在不同程度的结果。随着遗传学和药理学工具可用于测试单个基因在再生过程中的利益的作用，有必要制定一个强大的LIRD范式。这里我们描述一个LIRD协议，导致在杆和锥感光体，其中结合使用的两个以前建立LIRD技术的广泛和一致的损失。此外这个协议可以延长使用色素的动物，从而消除了需要保持白化背景LIRD研究的转基因株系的利息。

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			<td height="21" style="height:21px;">Glass Petri dish (150 x 20 mm)</td>
			<td>Sigma-Aldrich/Pyrex</td>
			<td>CLS3160152BO</td>
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			<td height="21" style="height:21px;">250 ml glass beaker</td>
			<td>Sigma-Aldrich/Pyrex</td>
			<td>CLS1000250</td>
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			<td height="21" style="height:21px;">4 L glass beaker</td>
			<td>Sigma-Aldrich/Pyrex</td>
			<td>CLS10004L</td>
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			<td height="21" style="height:21px;">Aluminum foil</td>
			<td>Fisher</td>
			<td>01-213-105</td>
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			<td height="21" style="height:21px;">250 W halogen lamps</td>
			<td>Workforce</td>
			<td>265-669</td>
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			<td>Aquaneering</td>
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			<td>Honeywell</td>
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			<td height="21" style="height:21px;">Aerator</td>
			<td>Tetra</td>
			<td>77853-900</td>
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			<td height="21" style="height:21px;">Thermometer</td>
			<td>Cole-Parmer</td>
			<td>YO-08008-58</td>
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			<td height="21" style="height:21px;">Bent forceps (5/45)</td>
			<td>World Precision Instruments</td>
			<td>504155</td>
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a novel light damage paradigm for use in retinal regeneration studies in adult zebrafish

光诱导的视网膜变性（LIRD）是常用的啮齿动物损伤杆和视锥细胞和斑马鱼。在成年斑马鱼，光感受器变性触发穆勒胶质细胞重新进入细胞周期和产生瞬态放大祖​​。这些前体细胞继续增殖，他们迁移到受损区域，他们最终产生新感光。目前，有两个广泛使用LIRD的范例，其中每个感光损失和相应的差异在再生反应在不同程度的结果。随着遗传学和药理学工具可用于测试单个基因在再生过程中的利益的作用，有必要制定一个强大的LIRD范式。这里我们描述一个LIRD协议，导致在杆和锥感光体，其中结合使用的两个以前建立LIRD技术的广泛和一致的损失。此外这个协议可以延长使用色素的动物，从而消除了需要保持白化背景LIRD研究的转基因株系的利息。

此前所述的光治疗方案相比，每一个人的LIRD方法。在暗处理成年的白化动物（ 图3-5），个人光疗法造成重大损失棒（ 图3）和锥感光器（ 图4）。然而，无论是单独的治疗方法主要损坏感光体在背侧视网膜一半，留下相对保护的光的治疗方法（ 图3和4）的腹侧视网膜。此外，相比与卤素灯处理，UV光处理损坏的视锥细胞在背视?...

Watch this Scientific Journal Video about A Novel Light Damage Paradigm for Use in Retinal Regeneration Studies in Adult Zebrafish at JoVE.com

A Novel Light Damage Paradigm for Use in Retinal Regeneration Studies in Adult Zebrafish

多个光损伤协议已损坏感光细胞，从而诱发成年斑马鱼视网膜的再生反应。此协议描述了一种改进的方法，可以用在着色的动物，并在整个视网膜杆和视锥细胞，损害绝大多数。

成年斑马鱼视网膜再生研究中使用的一种新型的光损伤范式

Light-induced retinal degeneration (LIRD) is commonly used in both rodents and zebrafish to damage rod and cone photoreceptors. In adult zebrafish, ...

Research

JoVE Journal

Neuroscience

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

运行轮活动在啮齿类动物的昼夜节律的记录和分析

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

科研

神经科学

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

视网膜神经节细胞逆行标记的成年斑马鱼与荧光染料

As retrograde labeling retinal ganglion cells (RGCs) can isolate RGCs somata from dying sites, it has become the gold standard for counting RGCs in RGCs ...

Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration

斑马鱼成人端脑的一刀，受伤：一个方法调查脊椎动物脑部神经发​​生与再生

Adult zebrafish have an amazing capacity to regenerate their central nervous system after injury. To investigate the cellular response and the molecular ...

The Use of Trace Eyeblink Classical Conditioning to Assess Hippocampal Dysfunction in a Rat Model of Fetal Alcohol Spectrum Disorders

使用的跟踪经典条件反射，评估胎儿酒精谱系障碍大鼠模型海马功能障碍

Neonatal rats were administered a relatively high concentration of ethyl alcohol (11.9% v/v) during postnatal days 4-9, a time when the fetal brain ...

Modeling Amyloid-&#946;42 Toxicity and Neurodegeneration in Adult Zebrafish Brain

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成年斑马鱼脑 Amyloid-β42毒性和神经的模型研究

Alzheimer's disease (AD) is a debilitating neurodegenerative disease in which accumulation of toxic amyloid-&#946;42 (A&#946;42) peptides leads to ...

M&#252;ller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

MÃƒÆ’Ã†'Ãƒâ€šÃ‚Â¼ller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

激光诱导的斑马鱼视网膜变性及再生模型中的 m ü ller 胶质细胞活化

A fascinating difference between teleost and mammals is the lifelong potential of the teleost retina for retinal neurogenesis and regeneration after ...

Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments

Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments

从成年斑马鱼中制备新鲜视网膜切片用于前体成像实验

The retina is a complex tissue that initiates and integrates the first steps of vision. Dysfunction of retinal cells is a hallmark of many blinding ...

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

用双光子激光消融神经种群及其对斑马鱼幼虫钙成像和行为记录的评价

To identify the role of a subpopulation of neurons in behavior, it is essential to test the consequences of blocking its activity in living animals. Laser ...

A Novel In Vitro Model of Blast Traumatic Brain Injury

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Traumatic brain injury is a leading cause of death and disability in military and civilian populations. Blast traumatic brain injury results from the ...

An Emerging Target Paradigm to Evoke Fast Visuomotor Responses on Human Upper Limb Muscles

唤起人类上肢肌肉快速视觉运动反应的新兴目标范式

To reach towards a seen object, visual information has to be transformed into motor commands. Visual information such as the object&#8217;s color, shape, ...