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JoVE Journal

Immunology and Infection

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Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

8.3K Views

11:18 min

March 21st, 2014

March 21st, 2014

8,284 Views

0:05

Title

1:49

Infection of Synchronized Young Adult Worms with Pseudomonas aeruginosa

4:44

Setting up the Wormsorter

6:19

Sorting Colonized vs Noncolonized Worms

8:28

Results: Colonization is Not Required for the Transcriptional Response to P. aeruginosa

10:19

Conclusion

The wormsorter facilitates genetic screens in Caenorhabditis elegans by sorting worms according to expression of fluorescent reporters. Here, we describe a new usage: sorting according to colonization by a GFP-expressing pathogen, and we employ it to examine the poorly understood role of pathogen recognition in initiating immune responses.

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