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DOI :
10.3791/51116-v
April 21st, 2014
Chapters
0:05
Title
2:47
Collection and Processing of Myocardial Samples
3:52
Washing and Digestion of Myocardial Chunks
6:43
Cell Resuspension and Ca2+ Readapation
8:28
Functional Evaluation of Isolated Cardiomyocytes
11:11
Results
13:16
Conclusion
心脏疾病的细胞基础上的现有知识大多依赖于对动物模型的研究。在这里,我们描述和验证了一种新的方法从人心肌小型手术样品得到单一可行的心肌细胞。人心肌细胞可用于电生理学研究和药物测试。
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