Name: isolation and functional characterization of human ventricular cardiomyocytes from fresh surgical samples
Uploaded: 2014-04-21T15:45:00
Description: Watch this Scientific Journal Video about Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples at JoVE.com

Dette arbejde blev støttet af EU (STREP projekt 241577 &quot;BIG HEART,&quot; 7. europæiske rammeprogram, KP), Menarini International Operations Luxembourg (AM), Telethon GGP07133 (KP) og Gilead Sciences (AM).

De eksperimentelle protokoller om humant væv blev godkendt af den etiske komité i Careggi University Hospital (2006 / 0.024.713; fornyet maj 2009). Hver patient gav skriftligt informeret samtykke. 1. Løsninger og udstyr Forberedelse Løsninger er beskrevet i tabel 1. Et forenklet rutediagram over celleisolering procedure findes i figur 1.. <table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td colspan="2"> Opløsni...

<ol>
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Vi har beskrevet og valideret en metode til at isolere levedygtige myocytes fra kirurgiske prøver af human ventrikulær myokardiet. Begyndende fra tidligere beskrevne protokoller, der var blevet anvendt til at isolerede celler fra atrielle kirurgiske prøver, en teknik til at muliggøre separation af enkelte levedygtige myocytter fra syge ventrikulære myocardium blev udviklet og finjusteret. Tidlige rapporter viste, at isolation af enkelte cardiomyocytter fra bidder af atrial og ventrikulær væv selektivt værdiforri...

Dissektion af elektrofysiologiske egenskaber af myokardiet er skredet markant efter udvikling af teknikker for enkelt hjerte-myocyt isolation. Nylige fremskridt i forståelsen af ​​hjerte Excitation Sammentrækning Kobling (EF-kobling) er også blevet gjort muligt ved evnen til at isolere levedygtige enkelt cardiomyocytter, der bevarer alle de fysiologiske egenskaber af intakt væv. Patch clamp metoder rutinemæssigt anvendes til at studere funktionen og farmakologisk modulation af hjerte sarcolemmal ionstrømme. Optagelser af intracellulære calcium dynamik med Ca 2 + følsomme farvestoffer er også regelmæssigt udføres på enkelte hjertemyocytter fra en række sunde og syge modeller, der giver vigtige oplysninger om fysiologi EF-Kobling samt på de patologiske ændringer i intracellulær Ca 2 + homeostase fører til mekanisk svækkelse og øget arytmogene byrde i hjertesygdomme. Information fra disse studier er afgørende for at forstå de elektrofysiologiske og mekaniske virkninger af lægemidler i kliniske omgivelser. Men der er artsspecifikke forskelle i transmembrane strømninger og i EF-Hunstik proteiner, der tegner sig for specifikke træk ved hjertets handling potentiale og hjerte mekanik. Således, mens undersøgelser af myocytter isoleret fra ikke-humane pattedyr har belyst de biofysiske egenskaber og fysiologiske roller specifikke transmembrane ionkanaler og EF-Hunstik proteiner, har de ikke nødvendigvis relevante modeller for humane hjertemyocytter. Isolering af levedygtige myocytes fra menneskelig myokardiet er derfor vigtigt at forstå patofysiologien af ​​hjertesygdomme og validere nye terapeutiske tilgange. Humant atrialt væv er let tilgængelig som atriale vedhæng ofte kasseres under kirurgiske procedurer. Indledende kvantitative undersøgelser af voksne hjerte-virkningspotentialer menneskelige og ioniske currents ansat enzymatisk isolerede atrielle celler 1-4. Optagelser af handling potentialer eller strømninger fra isolerede voksne humane ventrikulære celler er efterfølgende blevet rapporteret 3,5-10. De fleste af disse undersøgelser har anvendt celler fra eksplanterede hjerter og anvendt enten collagenase perfusion af en koronararterie segment eller eksponering af relativt store mængder af udskåret væv collagenase til opnåelse af isolerede celler. Disse undersøgelser have en detaljeret karakterisering af et antal transmembrane ionstrømme fra humane ventrikulære kardiomyocytter fra sunde hjerter og fra patienter med terminal hjertesvigt. Optagelser af L-typen Ca2 + strøm (I Ca-L) 5-7, transient udadgående kaliumstrøm (I) 8, aktiv ensretter kalium (I κ1) 8, de forskellige komponenter af forsinkede ensretter kalium (I κ ) 9 er blevet rapporteret. Forskud og raffinering afisolation procedure 10, gav en klar karakterisering af den ioniske grundlag af den øgede arrhythmogene potentiale i terminal hjertesvigt, omfattende virkningspotentiale forlængelse 11, forsinket efter depolariseringer 12 og øget sjov strøm 13, der fører til diastolisk depolarisering og præmature beats. Voksen hjertemyocytter normalt isoleres fra små dyr ved retrograd perfusion af hele hjertet med forskellige enzymblandinger, en teknik, der producerer høje udbytter af Ca 2 +-tolerante celler 14. Isolering af hjertemyocytter fra fragmenter af væv i sig selv er mindre vellykket sandsynligvis på grund af den begrænsede adgang af enzymer til individuelle myocytter sammenlignes med den, der opnås ved perfusion af koronararterier. På grund af den meget begrænsede tilgængelighed af ubrugte donorhjerter, den eneste praktiske måde at opnå normale humane ventrikulære celler på en regelmæssig basis er ved enzymatisk digestion af de ofte meget små væv fragmenter skåret under elektive kirurgiske procedurer. Den eneste sygdomsmodel menneske, som er blevet grundigt karakteriseret på celleniveau er terminal hjertesvigt på grund af adgang til transplanterede hjerter. Forekommer imidlertid terminal hjertesvigt hos et mindretal af patienterne og ofte indebærer en fælles vej af alvorlig ombygning af myokardieceller, hvilket er relativt uafhængig af den underliggende årsag 15. Evnen til at vurdere funktionen af ​​enlige cardiomyocytes fra patienter på et tidligere ikke undlade stadium af sygdommen er afgørende at forstå den specifikke patofysiologi forskellige nedarvede eller erhvervede tilstande. Hypertrofisk kardiomyopati (HCM) er et sigende eksempel. HCM er en almindelig (1/500 individer) arvelig hjertesygdom karakteriseret ved hjertehypertrofi, øget arytmogene risiko-og kontraktile ændringer på grund af udstrømning tarmkanalen obstruktion og diastolisk dysfunktion 16. Kardiomyocytter fra HCM hjerter undergo en kompleks remodeling processer, der involverer ændringer i cellestruktur (hypertrofi, myofibrillar uorden) og EF-Kobling 17. Imidlertid har de fleste oplysninger om myocyt dysfunktion i HCM kommer fra transgene dyremodeller. Da kun et mindretal af HCM patienter udvikler mod terminal hjertesvigt og kræver hjertetransplantation, er meget sjældent tilgængelige for celleisolering med standardmetoder HCM hjerter. Men mindst 30% af HCM patienter udvikler obstruktive symptomer på grund af massiv septal hypertrofi ændre udstrømning tarmkanalen blodgennemstrømning under systole (HCM) 18. Den mest effektive tilgængelige terapeutisk mulighed for lindring af obstruktion i HCM er kirurgisk septal myectomy: I løbet af denne kirurgiske procedure, er en variabel størrelse del af øvre septum fjernes ved trans aorta tilgang. Denne del af hypertrophied septum er derfor tilgængelig for celle isolation fra frisk væv. En fremgangsmåde til isolering af humant ventricular myocytter fra enkelte, små transvenøse endomyokardiel biopsipræparater tidligere har været udviklet og udgivet 19. Vi implementerede en metode til at isolere enkelte septumdefekter myocytes fra ventrikulære myocardium prøver fra patienter, der gennemgår hjertekirurgi, herunder patienter med HCM gennemgår septal myectomy og patienter, der gennemgår ventil udskiftning procedurer. Ud over en detaljeret beskrivelse af isolation protokollen, repræsentativ elektrofysiologiske og Ca 2 + fluorescens målinger præsenteres, dokumentere levedygtigheden af de isolerede humane ventrikelmyocytter og gennemførligheden af patch clamp og intracellulære Ca 2 + studier.

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		<tr height="21">
			<td height="21" style="height:21px;width:495px;">Potassium phosphate monobasic (KH2PO4)</td>
			<td style="width:172px;">Sigma-Aldrich</td>
			<td style="width:139px;"><a href="http://www.sigmaaldrich.com/catalog/product/sigma/p9791?lang=it&amp;region=IT">P9791&#160;</a></td>
			<td style="width:255px;"></td>
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		<tr height="21">
			<td height="21" style="height:21px;">Magnesium sulfate heptahydrate(MgSO4 * 7H2O)</td>
			<td style="width:172px;">Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/m1880?lang=it&amp;region=IT">M1880&#160;</a></td>
			<td></td>
		</tr>
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			<td height="21" style="height:21px;">HEPES</td>
			<td style="width:172px;">Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/h3375?lang=it&amp;region=IT">H3375&#160;</a></td>
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			<td height="21" style="height:21px;">Adenosine</td>
			<td style="width:172px;">Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/a9251?lang=it&amp;region=IT">A9251&#160;</a></td>
			<td></td>
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			<td height="21" style="height:21px;">D-(+)-Glucose</td>
			<td style="width:172px;">Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/g8270?lang=it&amp;region=IT">G8270&#160;</a></td>
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			<td height="21" style="height:21px;">Mannitol</td>
			<td style="width:172px;">Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/m4125?lang=it&amp;region=IT">M4125&#160;</a></td>
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			<td height="21" style="height:21px;">Taurine</td>
			<td style="width:172px;">Sigma-Aldrich</td>
			<td>T0625</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Potassium hydroxide (KOH)</td>
			<td>Sigma-Aldrich</td>
			<td>P5958</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Sodium chloride (NaCl)</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/s7653?lang=it&amp;region=IT">S7653</a></td>
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		<tr height="21">
			<td height="21" style="height:21px;">Potassium chloride (KCl)</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/p9333?lang=it&amp;region=IT">P9333&#160;</a></td>
			<td></td>
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			<td height="21" style="height:21px;">Sodium phosphate dibasic (Na2HPO4)</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/s7907?lang=it&amp;region=IT">S7907&#160;</a></td>
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		<tr height="21">
			<td height="21" style="height:21px;">Sodium bicarbonate (NaHCO3)</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/s6297?lang=it&amp;region=IT">S6297&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Potassium bicarbonate (KHCO3)</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/237205?lang=it&amp;region=IT">237205</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Sodium pyruvate</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/p2256?lang=it&amp;region=IT">P2256&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">2,3-Butanedione monoxime</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/b0753?lang=it&amp;region=IT">B0753&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Sodium hydroxide(NaOH)</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/s8045?lang=it&amp;region=IT">S8045&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">L-Glutamic acid monopotassium salt monohydrate</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/fluka/49601?lang=it&amp;region=IT">49601</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Pyruvic acid</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/aldrich/107360?lang=it&amp;region=IT">107360</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">3-Hydroxybutyric acid</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/aldrich/166898?lang=it&amp;region=IT">166898</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Adenosine 5&#8242;-triphosphate dipotassium salt dihydrate (K2-ATP)</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/a8937?lang=it&amp;region=IT">A8937</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Creatine</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/c0780?lang=it&amp;region=IT">C0780&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Succinic Acid</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/s3674?lang=it&amp;region=IT">S3674&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Ethylene glycol-bis(2-aminoethylether)-N,N,N&#8242;,N&#8242;-tetraacetic acid (EGTA)</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sial/e0396?lang=it&amp;region=IT">E0396&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Albumin from bovine serum</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/a0281?lang=it&amp;region=IT">A0281</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Magnesium chloride (MgCl2)</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/m8266?lang=it&amp;region=IT">M8266&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Collagenase from Clostridium histolyticum, Type V</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/c9263?lang=it&amp;region=IT">C9263&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Proteinase, Bacterial, Type XXIV</td>
			<td>Sigma-Aldrich</td>
			<td>P8038</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Calcium chloride solution, ~1 M in H2O</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/21115?lang=it&amp;region=IT">21115</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Calcium chloride 0.1 M solution</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/53704?lang=it&amp;region=IT">53704</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Potassium methanesulfonate</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/aldrich/83000?lang=it&amp;region=IT">83000</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">FluoForte Reagent</td>
			<td>Enzo Life Sciences</td>
			<td>ENZ-52015</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Powerload concentrate, 100X</td>
			<td>Life Technologies</td>
			<td>P10020</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Perfusion Fast-Step System</td>
			<td>Warner Instruments</td>
			<td>VC-77SP</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Amphotericin B solubilized</td>
			<td>Sigma-Aldrich</td>
			<td><a href="http://www.sigmaaldrich.com/catalog/product/sigma/a9528?lang=it&amp;region=IT">A9528&#160;</a></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Multiclamp 700B patch-clamp amplifier</td>
			<td>Molecular Devices</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Digidata 1440A</td>
			<td>Molecular Devices</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">pClamp10.0&#160;</td>
			<td>Molecular Devices</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="108">
			<td height="108" style="height:108px;">Digestion Device</td>
			<td>CUSTOM</td>
			<td>CUSTOM</td>
			<td style="width:255px;">The device is custome made in our laboratory using plastic tubes, cast Sylgard and a motor; it is described in detail in Fig 1 C-D and in Fig.7. We can provide further details if requested</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Silicone elastomer for the digestion device&#39;s brushes</td>
			<td>Dow Corning</td>
			<td>SYLGARD&#174; 184</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Variable speed rotating motor for the digestion device</td>
			<td>Crouzet</td>
			<td>Crouzet 178-4765&#160;</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">Mold for brushes casting</td>
			<td>N.A.</td>
			<td>N.A.</td>
			<td style="width:255px;">The mold is custom made from standard PTFE 2.5 cm diameter rods</td>
		</tr>
	</tbody>
</table>

isolation and functional characterization of human ventricular cardiomyocytes from fresh surgical samples

Kardiomyocytter fra syge hjerter er udsat for komplekse remodeling processer, der involverer ændringer i cellestruktur, excitation sammentrækning kobling og membran ionstrømme. Disse ændringer vil sandsynligvis være ansvarlige for den øgede arytmogene risiko og de kontraktile ændringer, der fører til systolisk og diastolisk dysfunktion i hjertepatienter. Imidlertid har de fleste oplysninger om de ændringer af myocyt funktion i hjertesygdomme kommer fra dyremodeller. Her er vi beskrive og validere en protokol til at isolere levedygtige myocytes fra små kirurgiske prøver af ventrikulære myokardiet fra patienter, der gennemgår hjertekirurgi operationer. Protokollen er beskrevet i detaljer. Elektrofysiologiske og intracellulære calcium målinger er rapporteret at demonstrere muligheden for en række encellede målinger i human ventrikulære cardiomyocytter opnået med denne metode. Protokollen rapporterede hanre kan være nyttige for fremtidige undersøgelser af den cellulære og molekylære grundlag for funktionelle ændringer af det menneskelige hjerte i tilstedeværelsen af ​​forskellige hjertesygdomme. Endvidere kan denne metode anvendes til at identificere nye terapeutiske mål på cellulært niveau og for at teste effektiviteten af ​​nye forbindelser på humane cardiomyocytter med direkte translationel værdi.

Den ovenfor beskrevne metode blev anvendt til at karakterisere de funktionelle abnormiteter i cardiomyocytes isoleret fra interventricular septum af patienter med hypertrofisk kardiomyopati (HCM), som gennemgik myectomy drift, sammenlignet med ikke svigtende ikke hypertrofisk kirurgiske patienter 21. Resultaterne i dette afsnit stammer fra dette arbejde 21 og er her vist som et eksempel på, hvordan denne teknik kan bruges til at karakterisere ændringer af myocardial celle funktion i hjerte sygdom...

Watch this Scientific Journal Video about Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples at JoVE.com

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

Aktuel viden om den cellulære grundlag af hjertesygdomme meste afhængig af undersøgelser af dyremodeller. Her beskriver vi og validere en ny metode til at opnå en enkelt levedygtige cardiomyocytter fra små kirurgiske prøver af human ventrikulær myokardiet. Menneskelige ventrikelmyocytter kan bruges til elektrofysiologiske undersøgelser og narkotika testning.

Isolering og funktionel karakterisering af human Ventrikulær kardiomyocytter fra Fresh Kirurgiske Prøver

Cardiomyocytes from diseased hearts are subjected to complex remodeling processes involving changes in cell structure, excitation contraction coupling and ...

Research

JoVE Journal

Medicine

Isolation of Human Islets from Partially Pancreatectomized Patients

Investigations into the pathogenesis of type 2 diabetes and islets of Langerhans malfunction 1 have been hampered by the limited availability of type 2 ...

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Patient Afledt Cellekultur og isolering af CD133<sup&gt; +</sup&gt; Formodede kræftstamceller fra melanom

Despite improved treatments options for melanoma available today, patients with advanced malignant melanoma still have a poor prognosis for ...

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Isolering af kræft stamceller fra humane Prostata Cancer Prøver

The cancer stem cell (CSC) model has been considerably revisited over the last two decades. During this time CSCs have been identified and directly ...

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

Indsamling, Isolation, og flowcytometrisk analyse for Human endocervikale prøver

Despite the public health importance of mucosal pathogens (including HIV), relatively little is known about mucosal immunity, particularly at the female ...

Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

Vurdering af Sarcoplasmic Reticulum Calcium Reserve og intracellulære diastolisk Calcium fjernelse i isolerede ventrikulær Cardiomyocytes

Intracellular calcium recycling plays a critical role in regulation of systolic and diastolic function in cardiomyocytes. Cardiac sarcoplasmic reticulum ...

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy

Teknik, minimalt Invasive tværgående aorta konstriktion i mus til induktion af venstre ventrikel hypertrofi

Transverse aortic constriction (TAC) in mice is one of the most commonly used surgical techniques for experimental investigation of pressure ...

Isolation and Cultivation of Adult Rat Cardiomyocytes

Isolering og dyrkning af voksen rotte Cardiomyocytes

In an intact heart, adjacent cells influence adult cardiomyocytes. With the method of isolation and cultivation of adult cardiomyocytes, a precise ...

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Udvikling og funktionel karakterisering af Murine Tolerogenic dendritiske celler

The immune system operates by maintaining a tight balance between coordinating responses against foreign antigens and maintaining an unresponsive state ...

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

Isolering af atrieflimren Cardiomyocytes fra en rotte Model af metabolisk syndrom-relaterede hjertesvigt med bevarede uddrivningsfraktion

In this article, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes (ACMs) from a rat model of ...

Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization

Generation af ventrikulær-lignende HiPSC-afledte Kardiomyocytter og høj kvalitet celle præparater til calcium håndtering karakterisering

Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) provide a valuable human source for studying the basic science of calcium (Ca2+) ...