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7.2K Views
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09:27 min
January 17th, 2014
DOI :
10.3791/51151-v
Chapters
0:05
Title
1:34
Creating the Recombinant Vector
2:48
Recombinant Virus Generation
5:26
Plaque Assay: NO Selection
5:59
Purity of Viral Stocks: PCR Screening of Genomic DNA
7:23
Results: Recombinant Vectors and the VACV Genome
8:43
Conclusion
Summary
一過性の支配的選択の方法を使用して蛍光タグ付きタンパク質を同時に発現する組換えワクシニアウイルスの生成のための迅速かつモジュール的なプロトコルがここに記載されている。
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