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JoVE Journal

Neuroscience

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Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

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11:46 min

January 13th, 2014

January 13th, 2014

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0:05

Title

2:23

Preparing Spinal Cord Sections

4:21

Staining Spinal Cord Sections

7:20

Laser Capture Microdissection (LMD)

10:08

Results: Motor Neuron Isolation

11:07

Conclusion

High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal cord sections with Azure B in 70% ethanol. Sufficient RNA (~40-60 ng) is recovered from 3,000-4,000 motor neurons to allow downstream RNA analysis by RNA-seq and qRT-PCR.

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