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DOI :
10.3791/51173-v
•
9:57 min
January 6th, 2014
Chapters
0:05
Title
1:11
Overview of Isolating DNA from Polytene Chromosomes by Laser Capture Microdissection
2:07
Probe Preparation via Whole Genome Amplification
3:59
3D FISH on Whole-mount Ovarian Tissue
7:33
WGA Kit Yields and FISH Labeling
9:31
Conclusion
染色体の絵画は、細胞核の組織化や核型の進化を研究するのに役立つ方法です。ここでは、その場での2次元蛍光化および3次元蛍光(FISH)に使用される単一ポリテン染色体から特定の領域を分離および増幅するアプローチ を 示す。
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