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DOI :
10.3791/51211-v
•
13:01 min
June 23rd, 2014
Chapters
0:05
Title
2:11
Sample Preparation and Substrate Selection
3:08
Addition of Controls and Samples
4:34
Addition of Fluorescent Conjugate
6:08
Identification of Positive and Negative Results
7:52
Results: Cell Staining Patterns for HEp-2
11:23
Conclusion
间接免疫荧光(IIF)的测定法在传统上被用于抗核抗体(ANA)的人血清中的检测。这些抗体的存在可以在全身性自身免疫性风湿病(SARD)帮助诊断。该协议演示了如何有效地进行IIF技术,准确检测这些自身抗体。
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