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DOI :
10.3791/51211-v
•
13:01 min
June 23rd, 2014
Chapters
0:05
Title
2:11
Sample Preparation and Substrate Selection
3:08
Addition of Controls and Samples
4:34
Addition of Fluorescent Conjugate
6:08
Identification of Positive and Negative Results
7:52
Results: Cell Staining Patterns for HEp-2
11:23
Conclusion
間接免疫蛍光(IIF)のアッセイは伝統的に、ヒト血清中の抗核抗体(ANA)の検出のために使用されてきた。これらの抗体の存在が全身性自己免疫リウマチ性疾患(SARD)の診断を助けることができる。このプロトコルは、効果的に正確にこれらの自己抗体を検出するために、IIF法を実行する方法を示しています。
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