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DOI :
10.3791/51228-v
•
10:39 min
September 14th, 2014
Chapters
0:05
Title
1:37
Preparation of Mitochondria for Electron Cryo-tomography
3:14
Recording Tomographic Tilt Series
5:20
Creation and Segmentation of Tomographic Volumes
6:25
Subtomogram Averaging of ATP Synthase Dimers and Fitting of X-Ray Structures
8:54
Conclusion
7:05
Results: Analysis of Mitochondrial Electron Cryo-tomograms
私たちは、収集する方法のプロトコル全体ミトコンドリアのプロセス電子クライオ断層像を提示する。技術はネイティブ生体膜に大きな膜タンパク質複合体の構造、機能、および組織への詳細な洞察を提供しています。
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