Name: adaptation of semiautomated circulating tumor cell (ctc) assays for clinical and preclinical research applications
Uploaded: 2014-02-28T14:45:00
Description: Watch this Scientific Journal Video about Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications at JoVE.com

इस काम के कैंसर रिसर्च के ओंटारियो संस्थान (# 08NOV230), अभिनव के लिए कनाडा फाउंडेशन (# 13,199), प्रोस्टेट कैंसर कनाडा, जैनसेन कैंसर विज्ञान, लंदन रीजनल कैंसर कार्यक्रम, और जॉन और डोना ब्रिस्टल से दाता समर्थन के माध्यम से अनुदान द्वारा समर्थित किया गया (ALA के लिए) लंदन स्वास्थ्य विज्ञान फाउंडेशन. लेल एक फ्रेडरिक Banting और चार्ल्स सर्वश्रेष्ठ कनाडा ग्रेजुएट छात्रवृत्ति डॉक्टरेट पुरस्कार द्वारा समर्थित है. ALA एक CIHR नई अन्वेषक पुरस्कार और अनुसंधान और नवाचार के ओंटारियो मंत्रालय से एक प्रारंभिक शोधकर्ता पुरस्कार के द्वारा समर्थित है.

इस पांडुलिपि में वर्णित सभी मानव अध्ययन पश्चिमी विश्वविद्यालय के मानव रिसर्च एथिक्स बोर्ड द्वारा अनुमोदित प्रोटोकॉल के तहत बाहर किया गया. सभी पशु अध्ययनों पश्चिमी विश्वविद्यालय जानवरों के उपयोग उ?...

<ol>
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2004 में सीएसएस की शुरूआत के बाद से कई नए सीटीसी प्रौद्योगिकियों के विकास के बावजूद, इस तकनीक अभी भी आज बाजार पर केवल चिकित्सकीय अनुमोदित प्रौद्योगिकी है और इसलिए यह सीटीसी का पता लगाने और गणन के लिए वर्त?...

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			<td height="21" style="height:21px;width:267px;">REAGENTS</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">0.5M EDTA</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">Anti-human CD44-FITC</td>
			<td style="width:175px;">BD Pharmigen&#160;</td>
			<td style="width:161px;">555478</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">Anti-human CD44-PE</td>
			<td style="width:175px;">BD Pharmigen&#160;</td>
			<td style="width:161px;">555479</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">Anti-human HLA-AlexaFluor488</td>
			<td style="width:175px;">BioLegend</td>
			<td style="width:161px;">311415</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">Anti-mouse CD45-APC</td>
			<td style="width:175px;">eBioscience</td>
			<td style="width:161px;">17-0451-82</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">Bond Primary Antibody Diluent</td>
			<td style="width:175px;">Leica</td>
			<td style="width:161px;">AR9352</td>
			<td></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:267px;">CellSave Preservative Tubes</td>
			<td style="width:175px;">Veridex</td>
			<td style="width:161px;">952820 (20 pack)&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160; 79100005 (100 pack)</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">CellSearch CTC Control Kit</td>
			<td style="width:175px;">Veridex</td>
			<td style="width:161px;">7900003</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">CellSearch CTC Kit</td>
			<td style="width:175px;">Veridex</td>
			<td style="width:161px;">7900001</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">CellSearch CXC Control Kit</td>
			<td style="width:175px;">Veridex</td>
			<td style="width:161px;">7900018RUO</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">CellSearch CXC Kit</td>
			<td style="width:175px;">Veridex</td>
			<td style="width:161px;">7900017RUO</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">Instrument Buffer</td>
			<td style="width:175px;">Veridex</td>
			<td style="width:161px;">7901003</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:267px;">Streck Cell Preservative (aka CytoChex)</td>
			<td style="width:175px;">Streck</td>
			<td>213350</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;"></td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:267px;">EQUIPMENT</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">1 ml syringe</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">10 ml serological pipette</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:267px;">1000&#181;l pipette</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">1000&#181;l pipette tips</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">12 x 75mm flow tubes</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">200&#181;l gel loading tips</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:267px;">200&#181;l pipette</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">22 gauge needle</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">5 3/4&#34; disposible pasteur pipet</td>
			<td style="width:175px;">VWR</td>
			<td style="width:161px;">14672-200</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:267px;">5 ml serological pipette</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:267px;">Automated pipettor</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:267px;">Capillary Blood Collection Tube (EDTA)</td>
			<td style="width:175px;">BD Microtainer</td>
			<td style="width:161px;">365974</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">CellSearch Analyzer II</td>
			<td style="width:175px;">Veridex</td>
			<td style="width:161px;">9555</td>
			<td rowspan="2" style="width:188px;">Includes magnests and verification cartirdges</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:267px;">CellSearch AutoPrep System</td>
			<td style="width:175px;">Veridex</td>
			<td style="width:161px;">9541</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:267px;">Centrifuge</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:267px;">MagCellect Magnet&#160;</td>
			<td style="width:175px;">R&#38;D Systems</td>
			<td style="width:161px;">MAG997</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:267px;">Small Latex Bulb</td>
			<td style="width:175px;">VWR</td>
			<td>82024-550</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:267px;">Vortex</td>
			<td style="width:175px;"></td>
			<td style="width:161px;"></td>
			<td></td>
		</tr>
	</tbody>
</table>

adaptation of semiautomated circulating tumor cell (ctc) assays for clinical and preclinical research applications

कैंसर से संबंधित मौतों के बहुमत metastatic रोग के विकास के लिए बाद में पाए जाते हैं. यह बेहद घातक बीमारी चरण ट्यूमर कोशिकाओं (CTCs) परिसंचारी की उपस्थिति के साथ जुड़ा हुआ है. इन दुर्लभ कोशिकाओं metastatic स्तन, प्रोस्टेट, और कोलोरेक्टल कैंसर में नैदानिक ​​महत्व का होना प्रदर्शित किया गया है. नैदानिक ​​सीटीसी का पता लगाने और गणन में वर्तमान सोने के मानक एफडीए को मंजूरी दे दी CellSearch प्रणाली (सीएसएस) है. इस पांडुलिपि मानक इस मंच द्वारा उपयोग प्रोटोकॉल के साथ ही रूपरेखा मानक का उपयोग कर, रोगी CTCs की प्रोटीन लक्षण वर्णन के लिए उपयोगकर्ता परिभाषित मार्कर अनुकूलन और खून की बहुत कम मात्रा में सीटीसी को पकड़ने के लिए एक तुलनीय प्रोटोकॉल की विस्तृत प्रक्रिया का वर्णन है कि दो अतिरिक्त अनुकूलित प्रोटोकॉल सीएसएस अभिकर्मकों, मेटास्टेसिस के विवो preclinical माउस मॉडल में अध्ययन के लिए. इसके अलावा, स्वस्थ दाता रक्त के बीच सीटीसी गुणवत्ता में अंतर मरीज रक्त samp बनाम टिशू कल्चर से कोशिकाओं के साथ नुकीलालेस डाला जाता है. अंत में, सीटीसी misclassification त्रुटियों के लिए नेतृत्व कर सकते हैं कि कई आमतौर पर प्रतिकूल आइटम रेखांकित कर रहे हैं. साथ में ले ली, इन प्रोटोकॉल मेटास्टेसिस और CTCs से संबंधित preclinical और नैदानिक ​​अनुसंधान में रुचि इस मंच के उपयोगकर्ताओं के लिए एक उपयोगी संसाधन प्रदान करेगा.

स्टैंडर्ड सीटीसी गणन परख सीएसएस की संवेदनशीलता और विशिष्टता अच्छी तरह से साहित्य में प्रलेखित किया गया है. हालांकि, बराबर सीटीसी वसूली को मान्य करने के लिए, नुकीला (1000 LNCaP मानव प्रोस्टेट कैंसर ...

Watch this Scientific Journal Video about Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications at JoVE.com

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications

परिसंचारी ट्यूमर कोशिकाओं (CTCs) कई metastatic कैंसर में शकुन हैं. इस पांडुलिपि सोने के मानक CellSearch प्रणाली (सीएसएस) सीटीसी गणन मंच और डाला आम misclassification त्रुटियों का वर्णन करता है. इसके अलावा, दो अनुकूलित प्रोटोकॉल इस तकनीक का उपयोग कर मेटास्टेसिस के preclinical माउस मॉडल में CTCs और सीटीसी गणन के उपयोगकर्ता परिभाषित मार्कर लक्षण वर्णन के लिए वर्णित हैं.

नैदानिक ​​और preclinical अनुसंधान अनुप्रयोगों के लिए ट्यूमर सेल (सीटीसी) Assays घूम semiautomated का अनुकूलन

The majority of cancer-related deaths occur subsequent to the development of metastatic disease. This highly lethal disease stage is associated with the ...

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Medicine

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

प्राथमिक ब्रेन ट्यूमर ऊतक के स्टेम सेल assays और फ्लो छंटनी के लिए प्रसंस्करण

Brain tumors are typically comprised of morphologically diverse cells that express a variety of neural lineage markers. Only a relatively small fraction ...

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

रोगी व्युत्पन्न सेल संस्कृति और CD133 का अलगाव<sup&gt; +</sup&gt; मेलेनोमा से ख्यात कैंसर स्टेम सेल

Despite improved treatments options for melanoma available today, patients with advanced malignant melanoma still have a poor prognosis for ...

In vivo 19F MRI for Cell Tracking

In vivo 19F MRI for Cell Tracking

सेल ट्रैकिंग के लिए विवो 19 एफ एमआरआई में

In vivo 19F MRI allows quantitative cell tracking without the use of ionizing radiation. It is a noninvasive technique that can be applied to humans. ...

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

एक उपन्यास<em&gt; में विवो</em&gt; जीन स्थानांतरण तकनीक और<em&gt; इन विट्रो</emMusculoskeletal विकारों में हड्डी हानि के अध्ययन के लिए&gt; सेल आधारित assays

Differentiation and activation of osteoclasts play a key role in the development of musculoskeletal diseases as these cells are primarily involved in bone ...

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

अलगाव, cryopreservation और संस्कृति नैदानिक ​​अनुप्रयोगों के लिए मानव भ्रूणावरण उपकला कोशिकाओं

Human amnion epithelial cells (hAECs) derived from term or pre-term amnion membranes have attracted attention from researchers and clinicians as a ...

Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics

Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics

प्राथमिक ट्यूमर और / या पूर्व नैदानिक ​​के लिए एसोसिएटेड Metastases और चिकित्सा के नैदानिक ​​विकास के पूर्व vivo उपचार की प्रतिक्रिया

The molecular analysis of established cancer cell lines has been the mainstay of cancer research for the past several decades. Cell culture provides both ...

Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model

एक माउस कर्क मॉडल से परिसंचारी ट्यूमर कोशिकाओं के अलगाव और प्रचार

Cancer metastasis is the foremost cause of cancer-associated deaths. Recent studies have shown that circulating tumor cells (CTCs) are important in cancer ...

Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells

प्रोस्टेट कैंसर के रोगी की पीढ़ी परिसंचारी ट्यूमर कोशिकाओं से xenograft मॉडल व्युत्पन्न

Patient derived xenograft (PDX) models are gaining popularity in cancer research and are used for preclinical drug evaluation, biomarker identification, ...

Proton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies

प्रोटॉन चिकित्सा वितरण और चयन ठोस ट्यूमर द्रोह में अपने नैदानिक आवेदन

Radiation therapy is a frequently used modality for the treatment of solid cancers. Although the mechanisms of cell kill are similar for all forms of ...

An In Vivo Method for Evaluating the Gut-Blood Barrier and Liver Metabolism of Microbiota Products

An In Vivo Method for Evaluating the Gut-Blood Barrier and Liver Metabolism of Microbiota Products

Microbiota उत्पादों की आंत-रक्त बाधा और जिगर चयापचय का मूल्यांकन करने के लिए Vivo विधि में एक

The gut-blood barrier (GBB) controls the passage of nutrients, bacterial metabolites and drugs from intestinal lumen to the bloodstream. The GBB integrity ...

नैदानिक ​​और preclinical अनुसंधान अनुप्रयोगों के लिए ट्यूमर सेल (सीटीसी) Assays घूम semiautomated का अनुकूलन

नैदानिक और preclinical अनुसंधान अनुप्रयोगों के लिए ट्यूमर सेल (सीटीसी) Assays घूम semiautomated का अनुकूलन