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11.9K Views
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10:03 min
August 10th, 2014
DOI :
10.3791/51310-v
Chapters
0:05
Title
2:03
Preparation of Hippocampal Slices
3:13
Baseline Recording and LTP Induction
5:17
Isolation of Nuclear Enriched Fractions from Hippocampal CA1
7:29
Semiquantitative Immunoblotting for Synapto-nuclear Proteins
8:02
Results: Induction of CA1 LTP Leads to the Accumulation of pJacS180 in the Nucleus
8:53
Conclusion
Summary
我们提供了用于诱导的长时程增强,在海马CA1区和核富集级分的从片的tetanized区域的随后分离的详细协议。这种方法可以被用来确定活性依赖的核蛋白质导入在学习和记忆的细胞模型。
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