Name: extracellular wire tetrode recording in brain of freely walking insects
Uploaded: 2014-04-01T16:30:00
Description: Watch this Scientific Journal Video about Extracellular Wire Tetrode Recording in Brain of Freely Walking Insects at JoVE.com

作者感谢尼克·凯斯曼的建议，并在准备稿件的帮助。这种技术是与由AFOSR下批FA9550-10-1-0054，并根据批准号：IOS-1120305美国国家科学基金会支持的快线RER工作联合开发。

1。四极管导线的制备<ol><li>拉出约1.1米长的一个非常薄的镍铬合金丝（12微米直径，PAC涂层）。附加的磁带标签，每一端。挂线在一个水平螺纹杆，使得两个端部在靠近台面相同的高度。 </li><li>重复步骤1.1为第二导线，使得总共4 2多个端部，并把它旁边的第一金属丝（约1厘米之间）。 </li><li>用胶带粘标签的四个两端连在一起，并在标签附加到一个电动旋转卷取装置。这种装置可以由廉价的直?...

<ol>
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而对昆虫大脑的CC或其他地区以前的电生理研究为我们提供了见解行为的控制中枢，他们大多在任内敛准备9,11或拴那些10,14分别进行。其结果是，在动物的感官体验和生理状态可以是从那些在自然的环境非常不同。此外，这种动物可以执行行为的任务是有限的，以一个平面下的那些情况。在这里，我们提出了一个方法，从自由活动的蟑螂消委会记录。但愿，我们已经为您提供了所?...

本文介绍了一个成功的系统，从内部的中央复杂的蟑螂，Blaberus discoidalis的（CC）的神经元记录，如昆虫走在舞台上，并处理导致它转身，隧道下，或翻越障碍的对象。导线也可以连接到一个激励器，以唤起活性在周围神经纤维与随后的行为变化。 在过去的十年中相当大的关注已经直指在控制昆虫行为的不同脑区所扮演的角色。这大部分的焦点已经指向被统称为中央复合体（CC）中线脑neuropils。已经取得进展，作为种类繁多的目标有关的行为对CC的作用问题技术的结果。这些技术包括从神经源性的操作，主要是在果蝇中 ，加上behavi口服分析1-3，该监测CC和尝试与该活动相关的行为上的参数范围内的神经活动电生理技术。 电生理技术，包括细胞内记录的个人识别4-9的神经元和细胞外记录，常与多通道探头10,11。这两种技术都是免费的。细胞内记录用锋利的电极或全细胞膜片提供鉴定神经元非常详细的数据，但仅限于一个或两个细胞一次，需要限制或无运动，能维持的时间相对较短。细胞外记录可以很容易地设置，不要求限制，并且可以保持几个小时。随着多通道四极管和集群切割，神经元的相当大的群体可以同时9,12分析。而整个细胞PATCH有栓系昆虫13被成功地使用，我们觉得也有必要的技术，使我们能够在大脑中记录神经活动的时间在自由活动的昆虫很长时间，因为他们对付障碍向前运动。 记录作为昆虫的动作和反弹向上和向下的需要推动我们朝着细胞外记录的方法。我们有良好的成功记录在内敛的准备与市售的16个通道的硅探针11，但即使是大蟑螂的小尺寸意味着该探测器必须安装脱体。也就是说，加上探针尖头的美味，使他们不适合免费步行的准备。在前面两个项目中，我们使用的细线形成四极管来完成类似的记录特性捆绑，但在一个更强大的安排。这些四极束使我们能够从束缚蟑螂录制ð消委会有关单位活动的变化，行走速度14和转向从一个杆10触角接触产生的行为。 很有用，因为这些束缚准备一直并将继续是，他们的确存在一定的局限性。首先，该昆虫可以执行的行为是仅限于一个平面上。也就是说，我们可以很容易唤起改变行走速度或转弯，但登山和隧道的行动是不可能的，至少具有典型的系绳安排。其次，我们的系留的准备工作“开环”。也就是说，它们不允许正常运动相关的反馈的系统。因此，当蟑螂开启我们的系绳，其视觉世界并没有相应地改变。这是不可能建立闭环系绳系统引入这种反馈。然而，它们是由模拟的视觉环境的编程和硬件的复杂性的限制。 Nevertheles秒，我们觉得我们可以改善我们现有拴记录方法从动物记录，因为它在舞台上或轨道和遇到的对象，因为它会在其自然环境自由地走了。 虽然无线系统，用于记录大脑活动15将是理想的，当前的系统中有记录的信道数，数据采集，电池寿命和重量的时间限制。因此，我们选择了尽量去适应我们的使用系留记录系统中自由移动的准备工作。为更好的无线系统变得可用，这种技术可以很容易地适应于这样的设备。本文中所描述的系统具有重量轻，工作得很好，似乎对蟑螂的行为有点不利的影响。与廉价的高速摄像头和簇切割软件，在个别大脑神经元的活动可能与运动。在这里，我们描述了prepar四极导线和植入昆虫的大脑，以及录音技术的电活动和运动，以及这些数据的振动性可汇聚供后续分析。

<table border="0" cellpadding="0" cellspacing="0" width="728">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:207px;">Nichrome wire&#160;</td>
			<td style="width:179px;">Sandvik Heating Technology</td>
			<td style="width:137px;">Kanthal RO-800</td>
			<td style="width:205px;">Use for tetrode</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Biomedical polyethylene tubing</td>
			<td>A-M Systems</td>
			<td>800700</td>
			<td>Use for tetrode tubing</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Lynx-8</td>
			<td>Neuralynx</td>
			<td></td>
			<td>Use for multi-unit recording</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Cheetah 32</td>
			<td>Neuralynx</td>
			<td></td>
			<td>Use for multi-unit recording</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">High speed camera</td>
			<td>Basler</td>
			<td>A602f</td>
			<td>Use fir video recording for walking experiments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">High speed camera</td>
			<td>Casio</td>
			<td>EX-FC150&#160;</td>
			<td>Use for video recording for climbing experiments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">WINanalyze</td>
			<td>Winanalyze</td>
			<td>version 1.4 3D</td>
			<td>Use for video tracking&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Matlab</td>
			<td>MathWorks</td>
			<td>MATLAB R2012b</td>
			<td>Use for TTL pulse generation and off-line data analysis</td>
		</tr>
	</tbody>
</table>

extracellular wire tetrode recording in brain of freely walking insects

大脑活动的昆虫电机控制中的作用越来越大的兴趣，需要，我们能够监视的神经活动，而昆虫进行自然的行为。我们以前开发的植入四极导线插入蟑螂的大脑，使我们能够从多个记录的神经元同时活动的中央复杂的技术，而拴蟑螂开启或改变行走速度。而一大进步，拴准备提供访问有限的行为，且往往缺乏发生在自由活动动物的反馈过程。我们现在提出的技术，使我们能够从自由移动蟑螂，因为他们走在舞台上，并通过旋转，爬坡或隧道处理障碍中心的复杂记录的修改版本。再加上高速视频和集群切割，我们现在可以与大脑活动的自由活动的昆虫运动的各种参数。

我们从27准备步行实验消委会录得50个单位的神经活动。 15那些准备（23单位），还进行攀岩实验。单个的单元是按照制剂和单元编号命名（ 例如，单元1-2表示制剂1，单元2）。 一个爬坡试验的视频快照， 如图4所示，整个视频可在补充视频1（声音从单元1-2）。录音是在正确的扇形体（FB）。蟑螂停下脚步，当它遇到的块，并用它的...

Watch this Scientific Journal Video about Extracellular Wire Tetrode Recording in Brain of Freely Walking Insects at JoVE.com

Extracellular Wire Tetrode Recording in Brain of Freely Walking Insects

我们以前开发的植入四极导线插入蟑螂大脑的中央复杂，使我们能够监视活动在拴蟑螂个别单位的技术。在这里，我们提出的技术，使我们能够也可自由移动的昆虫记录大脑活动的修改版本。

外导线四极管记录在行走自如昆虫大脑

Increasing interest in the role of brain activity in insect motor control requires that we be able to monitor neural activity while insects perform ...

Research

JoVE Journal

Neuroscience

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

逆行荧光染料标记允许有针对性的外单细胞记录技术鉴定神经元<em&gt;在体内</em

The overall goal of this method is to record single-unit responses from an identified population of neurons. In vivo electrophysiological recordings from ...

科研

神经科学

Electrophysiological Recording in the Brain of Intact Adult Zebrafish

在完整的成年斑马鱼大脑电生理记录

Previously, electrophysiological studies in adult zebrafish have been limited to slice preparations or to eye cup preparations and electrorentinogram ...

Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning

自由移动鸽子穿越三个阶段学习记录单个神经元&#39;动作电位

While the subject of learning has attracted immense interest from both behavioral and neural scientists, only relatively few investigators have observed ...

Recording EEG in Freely Moving Neonatal Rats Using a Novel Method

使用新颖的方法在自由移动的新生大鼠中记录脑电图

EEG is a useful method to detect electrical activity in the brain. Moreover, it is a widely used diagnostic tool for various neurological conditions, such ...

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

小容积回收-灌注-浸没式室系统中维持的急性海马切片的突触可塑性记录

Even though experiments on brain slices have been in use since 1951, problems remain that reduce the probability of achieving a stable and successful ...

Construction of an Improved Multi-Tetrode Hyperdrive for Large-Scale Neural Recording in Behaving Rats

一种改进的多电子四极超光速引擎在行为大鼠大尺度神经记录中的构建

Monitoring the activity patterns of a large population of neurons over many days in awake animals is a valuable technique in the field of systems ...

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

鼠脑切片偶联微电极阵列癫痫活性的记录与调控

Temporal lobe epilepsy (TLE) is the most common partial complex epileptic syndrome and the least responsive to medications. Deep brain stimulation (DBS) ...

Hybrid Microdrive System with Recoverable Opto-Silicon Probe and Tetrode for Dual-Site High Density Recording in Freely Moving Mice

混合微驱系统,带可回收的光电探头和Tetrode,用于自由移动小鼠的双位点高密度记录

Multi-regional neural recordings can provide crucial information to understanding fine-timescale interactions between multiple brain regions. However, ...

Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

多纤维光测量记录自由移动动物的神经活动

Recording the activity of a group of neurons in a freely-moving animal is a challenging undertaking. Moreover, as the brain is dissected into smaller and ...

Wireless Electrophysiological Recording of Neurons by Movable Tetrodes in Freely Swimming Fish

自由游泳鱼中可移动的特龙对神经元的无线电生理记录

The neural mechanisms governing fish behavior remain mostly unknown, although fish constitute the majority of all vertebrates. The ability to record brain ...