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12.1K Views
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08:57 min
March 10th, 2014
DOI :
10.3791/51346-v
Chapters
0:05
Title
1:42
Preparation of Bacterial Strains and Biparental Conjugation
4:42
Detection and Isolation of Mucoid Colonies
6:00
gDNA Restriction Digestion and Ligation and Inverse PCR and Sequence Analysis
7:07
Results: Mini-himar1 Mariner Transposon-mediated Mutagenesis
8:28
Conclusion
Summary
ここでは、画面に高密度の挿入変異体ライブラリーを生成するためのミニhimar1マリナートランスポゾンによる突然変異誘発を使用して、プロトコルを記述し隔離し、原型緑膿菌 PAO1株における新規アルギン酸レギュレータを識別します。
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