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DOI :
10.3791/51346-v
March 10th, 2014
Chapters
0:05
Title
1:42
Preparation of Bacterial Strains and Biparental Conjugation
4:42
Detection and Isolation of Mucoid Colonies
6:00
gDNA Restriction Digestion and Ligation and Inverse PCR and Sequence Analysis
7:07
Results: Mini-himar1 Mariner Transposon-mediated Mutagenesis
8:28
Conclusion
여기서 우리는, 화면 고밀도 삽입 돌연변이 체 라이브러리를 생성하기위한 미니 himar1 마리너 트랜스포존-매개 돌연변이 유발을 사용하여 프로토콜을 설명 분리 및 프로토 타입 녹농균 스트레인 PAO1 참신한 알긴산 레귤레이터를 식별한다.
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