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06:51 min
May 21st, 2014
DOI :
10.3791/51357-v
Chapters
0:05
Title
1:12
Mouse Heart Removal and Cannulation
2:54
Heart Perfusion and Digestion
3:25
Cells Dissociation and Calcium Reintroduction
5:10
Cell Culture
6:00
Results: Successful Isolation Quantification
6:29
Conclusion
Summary
We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.
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