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16.8K Views
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15:22 min
April 3rd, 2014
DOI :
10.3791/51385-v
Chapters
0:05
Title
2:30
Synthesis of the Modified Nucleoside Triphosphates
5:56
HPLC Purification of the Modified Nucleoside Triphosphates
7:40
Primer Extension Reactions and TdT Polymerizations
11:05
Results: Biochemical Characterization of Nucleoside Triphosphates
14:02
Conclusion
Summary
本文所描述的协议旨在解释和删节的复杂路线导致修饰的三磷酸核苷的方法的许多障碍。因此,该协议有利于激活这些构建模块两者的合成及其可用性的实际应用。
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