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DOI :
10.3791/51397-v
•
10:57 min
March 18th, 2014
Chapters
0:05
Title
1:26
Coating of Culture Inserts
2:08
Dissection of Hindbrains from E11.5 Mouse Embryos
5:40
Hindbrain Explant Culture
7:26
Wholemount Immunofluorescent Staining of Hindbrain Explants
9:00
Results: Neuronal Migration in Mouse Hindbrain in Ex Vivo Culture
10:28
Conclusion
胚胎神经元是出生在神经管的脑室区,但迁移到达到适当的目标。面部branchiomotor(FBM)的神经元,研究神经细胞迁移的有用模型。本协议描述的小鼠胚胎hindbrains平片的体外培养研究,规范FBM迁移机制。
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