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JoVE Journal

Neuroscience

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Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

11.0K Views

10:57 min

March 18th, 2014

March 18th, 2014

11,018 Views

0:05

Title

1:26

Coating of Culture Inserts

2:08

Dissection of Hindbrains from E11.5 Mouse Embryos

5:40

Hindbrain Explant Culture

7:26

Wholemount Immunofluorescent Staining of Hindbrain Explants

9:00

Results: Neuronal Migration in Mouse Hindbrain in Ex Vivo Culture

10:28

Conclusion

Embryonic neurons are born in the ventricular zone of the neural tube, but migrate to reach appropriate targets. Facial branchiomotor (FBM) neurons are a useful model to study neuronal migration. This protocol describes the wholemount ex vivo culture of mouse embryo hindbrains to investigate mechanisms that regulate FBM migration.

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