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DOI :
10.3791/51410-v
•
12:42 min
July 2nd, 2014
Chapters
0:05
Title
1:50
HUVEC Layer Preparation
3:07
Purified Ligand Coating
5:11
Neutrophil Separation
8:33
Flow Chamber Adhesion Assay
10:20
Results: The Flow Chamber Assay Enables Analyses of Adhesive Properties of Human Peripheral Blood Neutrophils to HUVECs and Purified Ligand Substrates
11:49
Conclusion
报道定量中性粒细胞粘附的方法。此方法创建类似于血管遇到了动态流环境。它使中性粒细胞粘附到任何纯化黏附分子(配体)或类似体内环境与剪切应力的上下文内皮细胞基板(HUVEC)的调查。
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