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Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

14.6K Views

12:48 min

March 25th, 2014

March 25th, 2014

14,625 Views

0:05

Title

1:30

Preparation of Antibody Coated Magnetic Beads and Extraction of Nuclei from Drosophila Larval Tissues

4:16

Collection of Pre-isolation Samples

6:16

Isolation of GFP-tagged Nuclei from Drosophila Larval Tissues

8:45

Results: Analysis of Tagged-nuclei Enrichment "Pre-isolatoin" and "Post-isolation" Samples

12:28

Conclusion

Drosophila tissues often contain a heterogeneous mixture of cell types. To examine gene expression in specific cell types from a particular tissue, nuclei can be genetically tagged and subsequently isolated using an affinity-based approach. Isolated nuclei can be used for downstream applications such as gene expression analysis and chromatin immunoprecipitation.

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