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14.2K Views
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15:12 min
May 10th, 2014
DOI :
10.3791/51447-v
Chapters
0:05
Title
1:46
Solubilization of Microsomes
3:49
Nickel-affinity Purification of CFTR
5:33
Gel Permeation Chromatography (GPC) Purification of CFTR
6:57
Reconstitution of CFTR
7:51
Measurement of ATPase Activity
9:35
Results: Isolation of Milligram Quantities of CFTR by Two Methods
14:28
Conclusion
Summary
异源表达和囊性纤维化跨膜电导调节器(CFTR)的净化是显著的挑战和制约因素的药物治疗囊性纤维化的发展。这个协议描述了两种方法的CFTR的毫克量适合于功能和结构研究的分离。
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