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14.2K Views
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15:12 min
May 10th, 2014
DOI :
10.3791/51447-v
Chapters
0:05
Title
1:46
Solubilization of Microsomes
3:49
Nickel-affinity Purification of CFTR
5:33
Gel Permeation Chromatography (GPC) Purification of CFTR
6:57
Reconstitution of CFTR
7:51
Measurement of ATPase Activity
9:35
Results: Isolation of Milligram Quantities of CFTR by Two Methods
14:28
Conclusion
Summary
異種発現および嚢胞性線維症膜コンダクタンス制御因子(CFTR)の精製は、重大な課題および嚢胞性線維症のための薬物療法の開発における制限因子である。このプロトコルは、機能的および構造的研究に適しCFTRのミリグラム量の単離のための2つの方法について説明します。
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