Name: a low-cost method for analyzing seizure-like activity and movement in drosophila
Uploaded: 2014-02-19T15:30:00
Description: Watch this Scientific Journal Video about A Low-cost Method for Analyzing Seizure-like Activity and Movement in Drosophila at JoVE.com

作者还希望表达感谢克里斯刻录机，斯蒂芬·麦金尼，劳拉·托宾，珍妮Gilbreath，阿什利日Olley，梅根·奥费，以及梅根海德他们在微调工作，此法。

1。苍蝇的步态分析个人的制备<ol><li>转让苍蝇轻轻拍打成单独的空瓶子和盖子的小瓶用棉塞。让苍蝇品静置20-30分钟观察之前。它不麻醉前行为观察的时间内苍蝇与以前的研究发现麻醉曝光可以改变的行为相比，非麻醉苍蝇14是非常重要的。 </li><li>轻轻拍打飞出来的小瓶，然后将个别苍蝇在一个直径为5厘米的培养皿盖（5毫米高）的小缝隙，让空气通过。从下面照射并利用安装在上面的?...

<ol>
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R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+feeding+behaviour+and+locomotor+activity+by+takeout+in+Drosophila.">Regulation of feeding behaviour and locomotor activity by takeout in Drosophila.</a> J. Exp. Biol. 210, 1424-1434 (2007).</li><li>Koudounas, S., Green, E. W., Clancy, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reliability+and+variability+of+sleep+and+activity+as+biomarkers+of+ageing+in+Drosophila.">Reliability and variability of sleep and activity as biomarkers of ageing in Drosophila.</a> Biogerontology. 13, 489-499 (2012).</li><li>Catterson, J. H., Knowles-Barley, S., James, K., Heck, M. M., Harmar, A. J., Hartley, P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+modulation+of+Drosophila+sleep-wake+behaviour.">Dietary modulation of Drosophila sleep-wake behaviour.</a> PLoS One. 5, (2010).</li><li>Donelson, N., Kim, E. Z., Slawson, J. B., Vecsey, C. G., Huber, R., Griffith, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-Resolution+positional+tracking+for+long-term+analysis+of+Drosophila+sleep+and+locomotion+using+the+''Tracker''+program.">High-Resolution positional tracking for long-term analysis of Drosophila sleep and locomotion using the ''Tracker'' program.</a> PLos ONE. 7, (2012).</li><li>Duboff, B., Fridovich-Keil, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mediators+of+a+long-term+movement+abnormality+in+a+Drosophila+melanogaster+model+of+classic+galactosemia.">Mediators of a long-term movement abnormality in a Drosophila melanogaster model of classic galactosemia.</a> Dis. Model Mech. 5, 796-803 (2012).</li><li>Seiger, M. B., Kink, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+anesthesia+on+the+photoresponses+of+four+sympatric+species+of+Drosophila.">The effect of anesthesia on the photoresponses of four sympatric species of Drosophila.</a> Behav. Genet. 23, 99-104 (1993).</li><li>Pavlidis, P., Ramaswami, M., Tanouye, M. 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当检查的运动或运动模式在果蝇中 ，它才能够提取关于移动的距离信息，运动的速度和运动模式是有用的。为了提取这些信息，昂贵的设备在传统上一直采用的往往是望而却步那些希望谨慎4,8,9使用这样的检测更小的实验室或实验室。 此处所描述的测定法可用于确定行驶的距离（ 图2和6）的运动，速度（ 图4和7）</st...

鉴于其寿命短和可用的健壮的遗传工具， 果蝇是用于研究各种疾病的病因学和各种生物过程中的潜在生理学极好的模型系统。在许多情况下，有利的是测量的影响，行为，遗传或药理操作对运动在这些模型有机体1,2。 有很多种方法，这些方法通常用于测量在两维3-7苍蝇的运动。这些系统可同时支持多个苍蝇的跟踪，并且可以测量速度，记录的路径长度，并记录时间的苍蝇已经使用了移动的百分比。它们已被用于研究在各种情况下，包括药物对运动和苍蝇的运动6-9的性二态性质的影响的运动。这些系统的主要缺点是跟踪系统的成本统或相应的软件和照相机。在某些情况下，这可以在数千美元运行。成本是一个实验室，将只需要有限的使用这样的系统，例如，量化新分离的突变体的机车图案的特别关注。 一个更简单的，但不太可靠的方法是使用其中记录的运动的基础上多少次穿过蝇被放置在闭合管10,11的中途的红外光束路径的系统。虽然这种系统可以提供运动和睡眠 - 觉醒周期的有价值的信息，他们可以超过或低于估计的运动，因为他们无法捕捉苍蝇的实际路径。例如，苍蝇，表现出在管的端部相当的运动将登记为低运动苍蝇虽然额外高分辨率的方法已被用来尝试规避这些限制12。 简单，便宜还都爬不动了测量geotaxi设备秒，苍蝇的向上运动，通过单管或一系列管2,13的。虽然这样的系统很便宜，可以很容易地识别趋地性的缺陷，他们无法捕捉到许多其他方面的运动，将感兴趣的研究者。 对于许多实验室，低成本的强大的分析系统，该系统简单的设置和操作将是一个有利的工具表征果蝇品系行为上的差异。在这里，我们描述了可以设置为不到两百元，并能提供有关的路径，速度苍蝇的运动和时间信息的检测。以证明该测定的功效，我们提出的数据表明，它可用于标识：1）在一个爆炸敏感（BS）突变体易受癫痫发作和药物二甲双胍2）的能力，一个自发的缺陷而通常用于治疗II型糖尿病，以减少发作样活性（SLA）Ⅰ的强度n两个学士学位突变体。

<table border="0" cellpadding="0" cellspacing="0" style="width:1117px;" width="1117">
	<colgroup>
		<col />
		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="68">
			<td height="68" style="height:68px;width:251px;">Name of the Reagent/Equipment</td>
			<td style="width:152px;">Company</td>
			<td style="width:169px;">Catalog Number/Model</td>
			<td style="width:545px;">Comments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">WebCam</td>
			<td>Logitech</td>
			<td>Pro900</td>
			<td>Any quality webcam will suffice.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">HandiAVI time-lapse software</td>
			<td>Azcendant software</td>
			<td></td>
			<td>Latest version can be found at http://www.azcendant.com/</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">ImageJ</td>
			<td>NIH</td>
			<td></td>
			<td>Latest version can be found at http://rsbweb.nih.gov/ij/download.html</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Multiracker Plug-In</td>
			<td>NIH</td>
			<td></td>
			<td>Latest version can be found at http://rsbweb.nih.gov/ij/plugins/index.html</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Vortexer</td>
			<td>VWR</td>
			<td>Vortex Genie 2</td>
			<td>Most standard size vortexers such as the Vortex Genie 2 will suffice.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">5cm petri dish cover</td>
			<td>LabM Limited</td>
			<td>D011</td>
			<td>Smaller or larger petri dish covers can be used for an arena in movement assay.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Light box</td>
			<td colspan="2">Built from scrap material.</td>
			<td>Illumination is used for the locomotion assay. Depending on the room lighting, it is possible to perform the assay without the light box.&#160;</td>
		</tr>
	</tbody>
</table>

a low-cost method for analyzing seizure-like activity and movement in drosophila

视频跟踪系统已被广泛地用于分析果蝇的运动和检测各种异常的机车行为。虽然这些系统可以提供丰富的行为信息，这些系统的成本和复杂性可能是令人望而却步的许多实验室。我们已经开发出用于测量果蝇机车行为，并扣押运动低成本的检测。该系统使用网络摄像捕获，可以使用廉价的和免费软件的组合来跟踪移动的距离被处理的图像，运动和运动的持续时间在指定的时间范围的平均流速。为了证明该系统的效用，我们研究了D组的果蝇突变体，该邦敏感（BS）瘫痪，这比野生型果蝇3-10倍更易患癫痫样活性（SLA）。使用这种新颖的系统，我们能够检测到该BS的Mutan吨的一声毫无意义的（BSS）具有探索性的运动在一个新的环境比野生型果蝇较低水平。此外，该系统被用来确定该药物二甲双胍，其通常被用于治疗II型糖尿病，降低了SLA的在BS中的突变体的强度。

这里所描述的技术已被以前用来分析在果蝇突变体学士学位1 SLA差异。这里给出的结果涉及的BS菌株容易感到震惊（EAS），轰的一声毫无意义的（BSS），以及技术性击倒（TKO）。在EAS基因编码参与磷脂酰乙醇胺合成15乙醇胺激酶和将军澳基因编码线粒体riboprotein 16。该BSS突变是麻痹 （ 对 ）电压门控钠通道17的等?...

Watch this Scientific Journal Video about A Low-cost Method for Analyzing Seizure-like Activity and Movement in Drosophila at JoVE.com

A Low-cost Method for Analyzing Seizure-like Activity and Movement in Drosophila

在分析癫痫样活动和运动的一种低成本方法<em&gt;果蝇</em

使用自由和廉价的软件程序的网络摄像头和相结合的，在果蝇机车图案可以被分析以检测速度，距离，以及各马达的活动时间的差别。

Video tracking systems have been used widely to analyze Drosophila melanogaster movement and detect various abnormalities in locomotive behavior. While ...

Research

JoVE Journal

Neuroscience

A Low-cost Method for Analyzing Seizure-like Activity and Movement in Drosophila

Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

含量自发活动，研究昼夜节律和睡眠参数果蝇</em

Most life forms exhibit daily rhythms in cellular, physiological and behavioral phenomena that are driven by endogenous circadian (&equiv;24 hr) ...

科研

神经科学

A Low Cost Setup for Behavioral Audiometry in Rodents

在啮齿动物的行为测听的低成本设置

In auditory animal research it is crucial to have precise information about basic hearing parameters of the animal subjects that are involved in the ...

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

在自发活动的测定<em&gt;果蝇</em

Drosophila melanogaster has been used as an excellent model organism to study environmental and genetic manipulations that affect behavior. One such ...

A Human-machine-interface Integrating Low-cost Sensors with a Neuromuscular Electrical Stimulation System for Post-stroke Balance Rehabilitation

人机界面集成的低成本传感器，具有神经肌肉电刺激系统中风后康复平衡

A stroke is caused when an artery carrying blood from heart to an area in the brain bursts or a clot obstructs the blood flow to brain thereby preventing ...

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

测定冷灵敏度和适应小鼠一种简单廉价的方法

Cold hypersensitivity is a serious clinical problem, affecting a broad subset of patients and causing significant decreases in quality of life.  The cold ...

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila

方法对敌人造成闭合性颅脑创伤性脑损伤<em&gt;果蝇</em

Traumatic brain injury (TBI) affects millions of people each year, causing impairment of physical, cognitive, and behavioral functions and death. Studies ...

Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation

小鼠脑切片准备扣押样活性的直接电流刺激和多电极阵列记录

Cathodal transcranial direct-current stimulation (tDCS) induces suppressive effects on drug-resistant seizures. To perform effective actions, the ...

High-density Electroencephalographic Acquisition in a Rodent Model Using Low-cost and Open-source Resources

高密度的脑电图采集在啮齿动物模型使用低成本的开源资源

Advanced electroencephalographic analysis techniques requiring high spatial resolution, including electrical source imaging and measures of network ...

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

分析突触调制<em&gt;果蝇</em&gt;曝光时间延长后，光光感受器

The nervous system has the remarkable ability to adapt and respond to various stimuli. This neural adjustment is largely achieved through plasticity at ...

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats

急性<em&gt;体内</em&gt;麻醉大鼠超导途径局部场电位和多单位活动的电生理记录

Converging evidence shows that many neuropsychiatric diseases should be understood as disorders of large-scale neuronal networks. To better understand the ...