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7.9K Views
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08:57 min
July 30th, 2014
DOI :
10.3791/51551-v
Chapters
0:04
Title
1:42
Transforming the Plasmid into Yeast Cells
3:36
Visualizing Aip1p-GFP Protein Movement Using Total Internal Reflection Fluorescence Microscopy
6:57
Results: Quantification of Fluorescently Tagged Aip1p Movement in Wild Type and Mutant Cells
8:29
Conclusion
Summary
致病突变的肌动蛋白可以改变细胞骨架的功能。细胞骨架动力学是通过利用全内荧光显微镜荧光标记蛋白成像量化。作为一个例子,细胞骨架蛋白,Aip1p,已经改变了定位和运动在细胞中表达突变的肌动蛋白同种型,R256H。
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