Sign In

A subscription to JoVE is required to view this content.

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

15.7K Views

08:08 min

July 9th, 2014

July 9th, 2014

15,658 Views

0:05

Title

1:24

Processing FFPE GI Tissue Blocks

3:17

DNA Extraction from Tissue Scrolls

4:30

Quantitative PCR

6:39

qPCR Results on Control Tissues

7:41

Conclusion

This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More