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DOI :
10.3791/51604-v
•
6:36 min
July 17th, 2014
Chapters
0:05
Title
1:37
Dissection and Initial Deyolking of Embryo
2:26
Fine Deyolking of the Embryo
3:18
Mounting Embryo on a Glass Slide
4:23
Results: In situ Hybridization of Flat-mounted Embryos
5:58
Conclusion
斑马鱼的胚胎是发育生物学研究的一个很好的模型。胚胎发育过程中,斑马鱼发展与蛋黄质量,其中介绍的样品观察和分析,三维的挑战。本协议描述了如何创建原位 (WISH)染色斑马鱼胚胎标本整装二维平板安装的准备工作。
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