Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

2:57

Induction of Differentiation in Female Embryonic Stem Cells to Induce X Chromosome Inactivation

4:55

Fixation of Differentiated ES Cells for Subsequent DNA-RNA FISH Analysis

5:33

Labeling of DNA Probes for FISH Experiments

8:02

Permeabilization, Pre-treatment and Hybridization of Fixed Cells for Combined DNA-RNA FISH

11:04

Post-hybridization Washes and Antibody Mediated Detection of Probe

13:21

Results: X Chromosome Inactivation in Differentiating Female ES Cells is Visualized by Combined DNA-RNA FISH

14:57

Conclusion