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Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

26.1K Views

10:10 min

September 20th, 2014

September 20th, 2014

26,114 Views

0:05

Title

1:15

Preparation of Larvae

2:08

Coarse Dissection of Larvae

3:30

Fixation and Staining of Tissue with Antibodies

6:17

Fine Dissection of Eye-Antennal Disc Complexes and Mounting

8:11

Results: Dynamic Images Produced for In-depth Analysis

9:39

Conclusion

The adult structures of Drosophila are derived from sac-like structures called imaginal discs. Analysis of these discs provides insight into many developmental processes including tissue determination, compartment boundary establishment, cell proliferation, cell fate specification, and planar cell polarity. This protocol is used to prepare imaginal discs for light/fluorescent microscopy.

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