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JoVE Journal

Neuroscience

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Pareadas Grabaciones de células enteras en organotípicos de hipocampo rebanadas

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09:23 min

September 28th, 2014

September 28th, 2014

14,538 Views

0:05

Title

1:16

Making Hippocampal Slices

3:50

Handling Hippocampal Slices

5:11

Whole Cell Recordings in Hippocampal Slices

7:39

Synaptic Connectivity Between CA3 Pyramidal Neurons

9:00

Conclusion

Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.

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