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Uma Experimental e Bioinformática Protocolo de Análises de RNA-seq de fotoperiódicas diapausa no Tigre Asiático Mosquito,

DOI :

10.3791/51961-v

November 30th, 2014

November 30th, 2014

13,120 Views

1Department of Biology, Georgetown University, 2Insect Physiology Lab, EEOB, The Ohio State University

RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.

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Genetics

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