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DOI :
10.3791/52023-v
October 23rd, 2014
Chapters
0:05
Title
1:20
Organotypic Entorhino-hippocampal Slice Cultures
2:37
In Vitro Hypoxia and Oxygen-glucose Deprivation (OGD) with Reperfusion
4:28
Fixation and Immunostaining
5:47
Determination of Regional Vessel Density
6:22
Results: Neuronal Death and Blood Vessel Loss after Hypoxia or OGD
7:41
Conclusion
虚血性脳損傷の多くの局面を再現することができentorhino海馬器官型切片培養のためのプロトコルは、提示される。神経細胞の変化に加えて、神経脈管構造の変化を研究することによって、このプロトコルは、損傷後の神経組織内のプラスチック製の変化を研究するための汎用性の高いツールです。
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