October 13th, 2014
•RNA interference (RNAi)-based gene knockdown techniques are at the core of Tribolium research. Here, we provide an overview of our larval RNAi technique in Tribolium castaneum. Larval RNAi is a simple, but powerful technique that provides quick access to loss-of-function phenotypes, allowing researchers to study gene functions in diverse contexts.
Tags
Related Videos
In vitro Reconstitution of the Active T. castaneum Telomerase
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
Chromatin Isolation by RNA Purification (ChIRP)
Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans
Measurement of Larval Activity in the Drosophila Activity Monitor
Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
TChIP-Seq: Cell-Type-Specific Epigenome Profiling
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved