Murine In Utero Electroporation

Overview

In utero electroporation is an important technique for studying the molecular mechanisms that guide the proliferation, differentiation, migration, and maturation of cells during neural development. Electroporation enables the rapid and targeted delivery of material into cells by utilizing electrical pulses to create transient pores in cell membranes. Although electroporation has traditionally been used in in vitro studies, scientific advancements have now broadened its utilization to intact organs, such as those found in mouse embryos developing in utero.

This video will introduce the key principles behind in utero electroporation in addition to reviewing the basic surgical techniques required to access developing embryos within a pregnant rodent. Details of the injection and electroporation steps are provided along with important considerations for directing gene delivery to specific brain regions. Finally, neurobiological applications of in utero electroporation are presented, such as investigating how specific genes contribute to neural development and how connections form between developing neurons.

Procedure

In utero electroporation is a key technique for studying the molecular mechanisms that guide neurodevelopment. By injecting DNA to alter gene expression in specific regions of the developing rodent brain, researchers can study the proliferation, differentiation, migration, and maturation of neural cells in the context of their natural environment.

This video will introduce the key principles behind in utero electroporation, a basic overview on how to perform the technique, and its applications in

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Tags
MurineIn Utero ElectroporationNeurodevelopmentMolecular MechanismsGene ExpressionRodent BrainProliferationDifferentiationMigrationMaturationNeural CellsNatural EnvironmentElectroporation PrinciplesDNA InjectionCell MembranesElectric Field PositioningIntact OrgansMouse EmbryosPhysiological CuesAxon Development

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0:00

Overview

0:47

Electroporation: Principles and Technical Considerations

2:32

Preparing for In Utero Electroporation

3:21

Performing In Utero Electroporation

5:28

Applications

7:33

Summary

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