Name: synergetic use of neural precursor cells and self-assembling peptides in experimental cervical spinal cord injury
Uploaded: 2015-02-23T13:00:00
Description: Watch this Scientific Journal Video about Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury at JoVE.com

我们要感谢为这项工作的健康研究（CIHR）加拿大研究所的资金支持，Krembil家庭基金会的主席亥尔波特在神经修复和再生，菲利普和佩吉DeZwirek，和戈登姚明的贡献，图2 。克劳斯Zweckberger资金由从“德意志Forschungsgesellschaft”（DFG）的资助。

注：以下实验方案被批准为大学健康网络（多伦多，加拿大）的动物保护委员会，并按照既定的指导，关心和准备使用的动物保健加拿大议会实验动物的政策。 1.颈椎动脉瘤夹挫伤/压缩​​模式<ol><li>术前高压灭菌仪器和通过将仪器在70％的酒精浴保持无菌条件下整个surgcial过程中。 </li><li>麻醉Wistar大鼠（250-270克）与氧的结合（O 2），一氧化二氮（N 2 O）（...

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此协议已被开发，以使读者对大鼠进行颈椎损伤模型，并使用同的SAP与NPC促进子宫颈脊髓损伤后更好的恢复的组合的治疗方法。 特别是在相对于其他宫颈创伤模型，如在（半）-transection模型或重降和震荡模型，夹子挫伤/压缩​​模型代表两个主要病理生理机制创伤 - 挫伤和压缩在，因此，模拟最佳临床病症。虽然它是公认的用于在大鼠和小鼠胸椎7-11的用途，它最近?...

脊髓损伤的50％以上都与颈椎。在临床上设置两个主要病理生理学机制的描述：脊髓的初始挫伤，随后，引起骨骨折，出血或组织肿胀正在进行压缩。 动脉瘤夹挫伤/压缩​​模型模拟两者的病理生理机制：捕捉剪辑产生挫伤和削波的持续时间表示所述压缩元件，承认其在引起骨骨折，出血或组织临床设置中的压缩肿胀最后显著更长。所用动脉瘤夹由一环状弹簧保证精确和可重现的剪裁力修改。尤其是在比较半侧横断或挫伤模型，这种动脉瘤夹模型模拟最好的临床设置。虽然患者的胸部损伤截瘫，从大多数颈椎病患者注射吃亏uries是四肢瘫痪，完全依赖。颈髓的解剖结构，然而，示出相比于胸椎或腰椎显著的差异，并且因此，被寻址特别是在此协议。 髓内腔和疤痕组织的发展，是恢复和再生障碍。为了克服这些障碍，利用支架材料是一种很有前途的方法。自组装肽可直接注射到病灶的中心。在那里，他们组装成纳米纤维支架桥接腔，并通过减少炎症和组织瘢痕改善抑制环境。而刚性材料造成相当大的损害植入期间脊髓，流体肽可以安全且没有严重的额外伤害注入。 改善与自组装肽的抑制环境干细胞移植之前，因此，支持小区integration，分化最后，功能恢复，颈脊髓损伤后。

<table border="0" cellpadding="0" cellspacing="0" width="541">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:273px;">Name</td>
			<td style="width:268px;">Company</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Aneurysmal clip</td>
			<td>SharpTech</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Surgical microscope</td>
			<td>Leica</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micro injection system</td>
			<td>World Precision Instruments, Inc.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Small animal stereotaxic instrument</td>
			<td>David Kopf Instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hamilton syringe</td>
			<td>Hamilton company</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Subdural pumps</td>
			<td>Alzet osmotic micro pump 1007D</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Surgical instrument</td>
			<td>Fine Science tools</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Isoflurane USP</td>
			<td>Pharmaceutical Partners of Canada Inc.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">0.9% Sodium Chloride injection USP</td>
			<td>Baxter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">7.5% Povidone iodine</td>
			<td>Purdue Pharma</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">70% Isopropyl alcohol USP</td>
			<td>GreenField Ethanol Inc.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">QL6 SAP</td>
			<td>Covidien</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">0.4% Trypan blue</td>
			<td>Gibco</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Platelet-Derived Growth Factor (PDGF)</td>
			<td>Sigma</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Epidermal Growth Factor (EGF)</td>
			<td>Sigma</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Fibroblast Growth Factor (FGF)</td>
			<td>Sigma</td>
		</tr>
	</tbody>
</table>

synergetic use of neural precursor cells and self-assembling peptides in experimental cervical spinal cord injury

脊髓损伤（SCI）导致严重的神经功能障碍和心理，经济和社会后果的患者和他们的家庭。在临床上，脊髓损伤的50％以上的影响颈椎1。作为原发性损伤的结果，二次机制，包括炎症，细胞凋亡，和脱髓鞘级联发生最终导致组织结疤髓内腔2,3和发展。均代表物理和化学障碍细胞移植，集成和再生。因此，塑造抑制环境和桥接腔营造一个有利的环境对细胞移植和再生是一种很有前途的治疗目标4。这里，子宫颈癌的SCI的使用的动脉瘤夹挫伤/压缩​​模型进行说明。这种模式比其他实验模型更临床相关的，因为完全横断或电源线的破裂是罕见的。此外，在试比较n至重量下降模型，它特别损害鼻背列，则脊髓的圆周压缩出现有利的。夹子闭合力和持续时间可以调整，以达到不同的损伤程度。环形的弹簧有利于精确校准和夹子力恒定。在生理条件下，合成的自组装肽（SAP）的自组装成纳米纤维，因此，呼吁在SCI 5的应用程序。它们可以直接注射到病灶减少损坏的线。 SAP的具有生物相容性的结构支架架设桥梁髓内腔，因此，装备损坏的电源线用于再生治疗。 K2（QL）6K2（QL6）是由Dong 等一升。6引入相对于其他的肽的新型的SAP，QL6自我组装成β片在中性pH SCI后60.14天，急性期，SAP的后被注入到病变和神经前体细胞（NPC）的中心是麟蹄反恐执行局到相邻背列。为了支持细胞存活，移植是结合的生长因子通过渗透微泵连续7天连续硬膜下施用。

<html>当执行上述的过程中，将得到一个SAP的支架桥接所述腔，并提供一种改进的抑制环境的，较少的组织瘢痕和增加鼻咽癌存活。 图4示出了在获得的大鼠脊髓的纵剖面损伤部位6周SCI后4周QL6 SAP注入和NPC移植后。 QL6肽成功注入脊髓，聚集在震中和半影扩散rostro-尾端。电子显微镜成像进一步示出，在图5中，1％的组件（重量/体积）QL6肽内2小时在PBS中稀释溶液的纳米纤维支?...

Watch this Scientific Journal Video about Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury at JoVE.com

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

Treating cervical spinal cord injury with both self-assembling peptides (SAP) and neural precursor cells (NPC), together with growth factors, is a promising approach to promote regeneration and recovery. A contusion/compression aneurysm clip rat model of cervical SCI and combined treatment involving SAP injection and NPC transplantation is established.

在实验颈脊髓损伤协同使用神经前体细胞，并自组装肽的

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

Research

JoVE Journal

Medicine

Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury

椎管内针对肌萎缩性脊髓侧索硬化症和外伤性脊髓损伤颈腹角细胞移植

Respiratory compromise due to phrenic motor neuron loss is a debilitating consequence of a large proportion of human traumatic spinal cord injury (SCI) ...

科研

医学

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

在大鼠脊髓损伤后的急性和慢性触觉测试

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor

单侧颈脊髓损伤的挫伤模型，利用无限的地平线冲击

While the majority of human spinal cord injuries occur in the cervical spinal cord, the vast majority of laboratory research employs animal models of ...

Controlled Cervical Laceration Injury in Mice

控制宫颈裂伤损伤小鼠

Use of genetically modified mice enhances our  understanding of molecular mechanisms underlying several neurological disorders  such as a spinal cord ...

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment (RMCA) in Individuals with Chronic Spinal Cord Injury

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment RMCA in Individuals with Chronic Spinal Cord Injury

评价呼吸肌慢性脊髓损伤个人使用呼吸电机控制评估（RMCA）

During breathing, activation of respiratory muscles is coordinated by integrated input from the brain, brainstem, and spinal cord. When this coordination ...

A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity

颈脊髓损伤的小鼠模型研究后皮损呼吸神经可塑性

A cervical spinal cord injury induces permanent paralysis, and often leads to respiratory distress. To date, no efficient therapeutics have been developed ...

Controlled Cortical Impact Model for Traumatic Brain Injury

控制皮层影响模型创伤性脑损伤

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

无线电遥测系统，以监测大鼠脊髓横断和胚胎神经干细胞移植在心血管功能

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

在脊髓损伤的实验挫伤型号神经干细胞移植

Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can ...

Calibrated Forceps Model of Spinal Cord Compression Injury

脊髓压迫损伤的校准钳型

Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...