Name: synergetic use of neural precursor cells and self-assembling peptides in experimental cervical spinal cord injury
Uploaded: 2015-02-23T13:00:00
Description: Watch this Scientific Journal Video about Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury at JoVE.com

Vi vil gerne anerkende finansieringen støtte til dette arbejde fra de canadiske Institutes of Health Research (CIHR) til Krembil Family Foundation, Halbert Chair i Neural Repair og Regeneration, Phillip og Peggy DeZwirek, og Gordon Yao for bidraget figur 2 . Klaus Zweckberger blev finansieret af en bevilling fra &quot;Deutsche Forschungsgesellschaft&quot; (DFG).

BEMÆRK: Følgende forsøgsprotokol blev godkendt af pasning af dyr udvalg i University Health Network (Toronto, Canada) og er i overensstemmelse med den politik, der er etableret i vejledningen til pasning og anvendelse af forsøgsdyr udarbejdet af den canadiske råd af dyrepleje . 1. Cervikal aneurisme Clip kontusion / Kompression Model <ol><li> Før operation autoklave instrumenter og holde sterile forhold under hele surgcial procedure ved at sætte instrumenterne i en alkohol bad 70%. ...

<ol>
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Denne protokol er blevet udviklet for at gøre det muligt for læseren at udføre en cervikal skade model hos rotter og bruge en kombineret fremgangsmåde behandling med strukturtilpasningsprogrammerne og NPC&#39;ere fremmer bedre restitution efter cervikal SCI. Især i forhold til andre cervikale traumer modeller, såsom (hemi) -transection model eller vægten drop og hjernerystelse modeller klippet kontusion / komprimering model repræsenterer både store patofysiologiske traumer mekanisme...

Mere end 50% af rygmarvsskader er relateret til halshvirvelsøjlen. I kliniske omgivelser to store patofysiologiske mekanismer er beskrevet: Den indledende kontusion af rygmarven og derefter den igangværende kompression forårsaget af knoglebrud, blødninger eller væv hævelse. Aneurismet Clip kontusion / kompression model efterligner begge patofysiologiske mekanismer: snapper klippet producerer en blodudtrædning og varigheden af ​​klipning repræsenterer komprimering komponent, erkendt, at kompressionen i kliniske omgivelser som følge af knoglebrud, blødninger eller væv hævelse sidste betydelig længere. Den anvendte aneurisme clip modificeres af en ring fjeder sikrer nøjagtig og reproducerbar klipning kraft. Især i forhold til hemi-transection eller kontusion model, denne aneurisme clips model efterligner bedste kliniske omgivelser. Mens patienter med thorakale læsioner lider paraplegi fleste patienter med cervikal injuries er tetraplegiker og helt afhængig. Den anatomiske struktur af livmoderhalskræft ledningen, viser imidlertid betydelige forskelle i forhold til den thorakale eller columna lumbalis, og dermed behandles navnlig i denne protokol. Udviklingen af ​​intramedullære hulrum og væv ardannelse er hindringer for genvinding og regenerering. For at overvinde disse barrierer brug af stillads materiale er en lovende tilgang. Selvsamlende peptider kan injiceres direkte i epicentret for læsionen. Der samler de ind nano-fiber stilladser bro hulrummet og forbedre hæmmende miljøet ved at mindske inflammation og væv skræmmer. Mens stive materialer forårsage betydelig beskadigelse af rygmarv under implantation, kan væsken peptider injiceres sikkert og uden alvorlige yderligere skade. Forbedring af inhiberende miljø med selvsamlende peptider før stamcelletransplantation dermed understøtte celle integration, differentiering og endelig funktionel restitution efter cervikal rygmarvsskade.

<table border="0" cellpadding="0" cellspacing="0" width="541">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:273px;">Name</td>
			<td style="width:268px;">Company</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Aneurysmal clip</td>
			<td>SharpTech</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Surgical microscope</td>
			<td>Leica</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micro injection system</td>
			<td>World Precision Instruments, Inc.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Small animal stereotaxic instrument</td>
			<td>David Kopf Instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hamilton syringe</td>
			<td>Hamilton company</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Subdural pumps</td>
			<td>Alzet osmotic micro pump 1007D</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Surgical instrument</td>
			<td>Fine Science tools</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Isoflurane USP</td>
			<td>Pharmaceutical Partners of Canada Inc.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">0.9% Sodium Chloride injection USP</td>
			<td>Baxter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">7.5% Povidone iodine</td>
			<td>Purdue Pharma</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">70% Isopropyl alcohol USP</td>
			<td>GreenField Ethanol Inc.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">QL6 SAP</td>
			<td>Covidien</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">0.4% Trypan blue</td>
			<td>Gibco</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Platelet-Derived Growth Factor (PDGF)</td>
			<td>Sigma</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Epidermal Growth Factor (EGF)</td>
			<td>Sigma</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Fibroblast Growth Factor (FGF)</td>
			<td>Sigma</td>
		</tr>
	</tbody>
</table>

synergetic use of neural precursor cells and self-assembling peptides in experimental cervical spinal cord injury

Rygmarvsskader (SCI) forårsage alvorlig neurologisk svækkelse og psykologiske, økonomiske og sociale konsekvenser for patienter og deres familier. Klinisk mere end 50% af SCI påvirke halshvirvelsøjlen 1. Som en konsekvens af primær læsion forekommer en kaskade af sekundære mekanismer, herunder inflammation, apoptose og demyelinering endelig fører til væv ardannelse og udvikling af intramedullære hulrum 2,3. Begge repræsenterer fysiske og kemiske barrierer for celle transplantation, integration og regeneration. Derfor forme hæmmende miljø og bygge bro hulrum for at tilvejebringe gunstige miljø for celle transplantation og regenerering er en lovende terapeutisk target 4. Her er en kontusion / kompression model af cervikal SCI ved hjælp af en aneurisme clips beskrevet. Denne model er mere klinisk relevant end andre eksperimentelle modeller, da fuldstændig overskæring eller brud af ledningen er sjældne. Også i SAMMENLIGNINGn til vægten drop model, som især skader dorsum kolonner, periferisk kompression af rygmarven forekommer fordelagtig. Clip lukkekraft og varighed kan justeres for at opnå forskellige alvorligheden. En ring fjeder letter præcis kalibrering og konstans klip kraft. Under fysiologiske betingelser, syntetiske selvsamlende peptider (SAP) selvorganisere i nanofibre og dermed appellerer til anvendelse i SCI 5. De kan injiceres direkte ind i læsionen minimere beskadigelse af ledningen. Strukturtilpasningsprogrammerne er biokompatible strukturer opføre stilladser at slå bro intramedullære hulrum og dermed udstyre den beskadigede ledning for regenerative behandlinger. K2 (QL) 6K2 (QL6) er en roman SAP introduceret af Dong et en l. 6 I sammenligning med andre peptider, QL6 selv samler ind β-sheets ved neutral pH 6 .14 dage efter SCI, efter det akutte stadium, strukturtilpasningsprogrammerne injiceres i midten af ​​læsionen og neurale precursorceller (NPC) er InjeCTED i tilstødende dorsale kolonner. For at understøtte celleoverlevelse, er transplantation kombineret med kontinuerlig subduralt administration af vækstfaktorer ved osmotiske mikro pumper til 7 dage.

<html> Ved udførelse af den ovenfor beskrevne procedure, vil du få et SAP stillads bygge hulrum og tilbyde en forbedring af den hæmmende miljø, mindre væv skræmme og en stigning i NPC overlevelse. Figur 4 viser et længdesnit af en rotte rygmarv fås på læsionsstedet 6 uger efter SCI og 4 uger efter QL6 SAP injektion og NPC transplantation. QL6 peptider lykkedes injiceret i ledningen, fremkommet i epicentret og diffust Rostro-kaudalt i Penumbra. Elektronmikroskop billeddannelse viser yderligere, ...

Watch this Scientific Journal Video about Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury at JoVE.com

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

Treating cervical spinal cord injury with both self-assembling peptides (SAP) and neural precursor cells (NPC), together with growth factors, is a promising approach to promote regeneration and recovery. A contusion/compression aneurysm clip rat model of cervical SCI and combined treatment involving SAP injection and NPC transplantation is established.

Fordelagtig samordning af neurale precursorceller og selvsamlende peptider i Experimental Cervikal Rygmarvsskader

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

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Use of genetically modified mice enhances our  understanding of molecular mechanisms underlying several neurological disorders  such as a spinal cord ...

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Kontrolleret Cortical Impact Model for Traumatisk Hjerneskade

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Neural stamcelletransplantation i Eksperimentel Contusive Model for Rygmarvsskader

Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can ...

Calibrated Forceps Model of Spinal Cord Compression Injury

Kalibreret pincet Model af rygmarvskompression Skade

Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...