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10:31 min
January 20th, 2015
DOI :
10.3791/52139-v
Chapters
0:05
Title
2:09
Dissection
4:25
Fixation, Permeabilization, and Immunochemistry
6:12
Proximity Ligation Assay and Mounting
8:06
Results: Detection of Endogenous Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction
9:51
Conclusion
Summary
此协议演示如何接近结扎检测试剂盒可用于检测原位蛋白质-蛋白质相互作用的果蝇幼虫神经肌肉接头。利用这种技术,光盘大和胡立泰邵示,以形成一复合的突触后区域,预先通过共免疫沉淀鉴定的关联。
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