Sign In

A subscription to JoVE is required to view this content.

Isolatie van Myofibroblasten van muis en mens slokdarm

10.9K Views

11:18 min

January 18th, 2015

January 18th, 2015

10,851 Views

0:05

Title

1:22

Isolating Murine Esophagi

3:51

Isolating Murine and Human Esophageal Stromal Cells

6:58

Characterizing Murine and Human Esophageal Stromal Cells

9:23

Results: Morphology, Cytochemistry and Purity of Cultured Esophageal Stromal Cells

10:56

Conclusion

We present a protocol to generate primary cultures of murine and human esophageal stromal cells with a myofibroblast phenotype. Cultured cells have spindle shaped morphology, express α-SMA and vimentin, and lack epithelial, hematopoietic and endothelial cell surface markers. Characterized stromal cells can be used in functional studies of epithelial-stromal interactions.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More