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DOI :
10.3791/52230-v
December 3rd, 2014
Chapters
0:05
Title
1:28
Preparation of Protein Extracts from Cultured Cells
3:05
Preparation of Protein Extracts from Mouse Liver and Spleen
4:56
Synthesis of Radiolabeled IRE-containing RNA Probe
5:55
Purification of Radiolabeled IRE-containing RNA Probe
7:48
Electrophoretic Mobility Shift Assay (EMSA)
10:27
Results: The Iron-dependence of IRE/IRP Interactions
12:17
Conclusion
在这里,我们提出了一个协议分析RNA /蛋白质相互作用。电泳迁移实验(EMSA)是基于RNA的/蛋白复合物和游离RNA在天然凝胶电泳迁移差异上。通过使用放射性标记的RNA探针,RNA /蛋白质复合物可以通过放射自显影进行显现。
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