January 19th, 2015
•Electroporation was used to insert purified bacterial virulence effector proteins directly into living eukaryotic cells. Protein localization was monitored by confocal immunofluorescence microscopy. This method allows for studies on trafficking, function, and protein-protein interactions using active exogenous proteins, avoiding the need for heterologous expression in eukaryotic cells.
Tags
Related Videos
Invasion of Human Cells by a Bacterial Pathogen
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
Isolation of Functional Cardiac Immune Cells
Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved