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11.8K Views
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08:48 min
January 4th, 2015
DOI :
10.3791/52343-v
Chapters
0:05
Title
1:22
Preparation of Glass Coverslips for Polyacrylamide Gels
2:59
Preparation of Polyacrylamide Gels for Invadopodia Assays
5:09
Preparation of Extracellular Matrix for Invadopodia Assays
7:10
Results: Invadopodia and Traction Force Assays
8:24
Conclusion
Summary
在肿瘤微机械刚性起着通过增加侵袭伪足活性和肌动球蛋白收缩驱动恶性行为了至关重要的作用。用聚丙烯酰胺凝胶(部分分配),侵袭伪足和牵引力测定可以被用于研究癌细胞的侵入性和收缩特性响应于矩阵刚性。
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